Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

DOI: 10.1369/jhc.4B6391.2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weier, H.-U. G.
Right arrow Articles by Munné, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weier, H.-U. G.
Right arrow Articles by Munné, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 53 (3): 269-272, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Fluorescence In Situ Hybridization and Spectral Imaging Analysis of Human Oocytes and First Polar Bodies

Heinz-Ulli G. Weier, Jingly F. Weier, Maria Oter Renom, Xuezhong Zheng, Pere Colls, Aida Nureddin, Chau D. Pham, Lisa W. Chu, Catherine Racowsky and Santiago Munné

E.O. Lawrence Berkeley National Laboratory (H-UGW,JFW,CDP,LWC), Berkeley, California; Department of Obstetrics, Gynecology, and Reproductive Sciences (JFW), University of California, San Francisco, California; Reprogenetics, LLC (MOR,SM), South San Francisco, California; Reprogenetics, LLC (XZ,PC,SM), West Orange, New Jersey; and Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School (AN,CR), Boston, Massachusetts

Correspondence to: Dr. Heinz-Ulli Weier, Life Sciences Division, MS 74-157, E.O. Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720. E-mail: ulliweier{at}hotmail.com

We investigated the frequencies of abnormalities involving either chromosome 1, 16, 18, or 21 in failed-fertilized human oocytes. Although abnormalities involving chromosome 16 showed an age-dependent increase, results for the other chromosomes did not show statistically significant differences among the three age groups, <35 years, 35–39 years, and >39 years. The scoring of four chromosomes is likely to underestimate the true rate of aneuploid cells. Therefore, for a pilot study investigating a more-comprehensive analysis of oocytes and their corresponding first polar bodies, we developed a novel eight-probe chromosome enumeration scheme using fluorescence in situ hybridization and spectral imaging analysis. (J Histochem Cytochem 53:269–272, 2005)

Key Words: aneuploidy • oocytes • polar bodies • fertilization • cytogenetics • fluorescence in situ hybridization


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
C. M. van der Loos
Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging
J. Histochem. Cytochem., April 1, 2008; 56(4): 313 - 328.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005

 
Purchase HCS Short Course Manual on HCS site