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DOI: 10.1369/jhc.4B6398.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (3): 297-299, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Analysis of Cell-free Fetal DNA in Plasma and Serum of Pregnant Women

T.V. Zolotukhina, N.V. Shilova and E. Yu Voskoboeva

Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moscow, Russia

Correspondence to: T.V. Zolotukhina, Research Centre for Medical Genetics, Prenatal Diagnosis, Russian Academy of Medical Sciences, Moskvorechje 1, Moscow, 115478, Russia. E-mail.tvz{at}medgen.ru

Sixty blood samples from pregnant women during gestational weeks 9–28 were investigated. Cell-free fetal DNA was extracted from maternal plasma or serum to be detected by nested PCR for determination of fetal gender. The SRY gene as a marker for fetal Y chromosome was detected in 34/36 women carrying a male fetus. In 3/24 women carrying female fetuses, the SRY sequence was also detected. Overall, fetal sex was correctly predicted in 91.7% of the cases. Therefore, the new, non-invasive method of prenatal diagnosis of fetal gender for women at risk of producing children with X-linked disorders is reliable, secure, and can substantially reduce invasive prenatal tests.

(J Histochem Cytochem 53:297–299, 2005)

Key Words: fetal DNA • maternal serum and plasma • SRY • nested PCR


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