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DOI: 10.1369/jhc.4B6426.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (3): 337-339, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Trophoblasts Isolated from the Maternal Circulation : In Vitro Expansion and Potential Application in Non-invasive Prenatal Diagnosis

Esther Guetta, Liat Gutstein-Abo and Gad Barkai

Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel

Correspondence to: Esther Guetta, Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel 52621. E-mail: Esther.Guetta{at}sheba.health.gov.il

Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis. (J Histochem Cytochem 53:337–339, 2005)

Key Words: trophoblast cells • prenatal diagnosis • cell sorting • in vitro culture • fluorescence in situ • hybridization


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005