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DOI: 10.1369/jhc.4A6410.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (3): 345-350, 2005
Copyright ©The Histochemical Society, Inc.

A Monoclonal Antibody with Potential for Aiding Non-invasive Prenatal Diagnosis : Utility in Screening of Pregnant Women at Risk of Preeclampsia

Alejandra Fernández, Belén Prieto, Ana Escudero, Jack H. Ladenson and Francisco V. Alvarez

Servicio de Análisis Clínicos, Hospital San Agustín, Avilés, Spain (AF); Servicio de Bioquímica (BP,FVA) and Servicio de Ginecología (AE), Hospital Universitario Central de Asturias, Oviedo, Spain (AE); Division of Laboratory Medicine, Department of Pathology and Immunology, Washington University, St. Louis, Missouri (JHL); and Servicio de Bioquímica, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Spain (FVA)

Correspondence to: Francisco V. Alvarez, Servicio de Bioquímica, Hospital Universitario Central de Asturias, Celestino Villamil s/n, 33006, Oviedo, Asturias, Spain. E-mail: falvarez{at}arrakis.es

The development of a non-invasive method of prenatal diagnosis in maternal blood has been the goal of our investigations during the last years. We have developed several anti-CD71 monoclonal antibodies and optimized a protocol for the isolation of nucleated red blood cells (NRBC) from peripheral maternal blood. The enhanced traffic of fetal erythroblasts into the maternal circulation in preeclampsia has been investigated by several groups. In this study, we compared one of our antibodies, 2F6.3, with a commercial anti-CD71 antibody in blood samples from pregnant women suffering pregnancy-induced hypertension (PIH) and in a control group of pregnant women without clinical features suggestive of an increased risk of developing preeclampsia. The mAb 2F6.3, developed by our group, has succeeded in isolating a significantly higher number of erythroblasts with less maternal cell contamination than the commercial antibody in both women with PIH and in the control group (p<0.01; Wilcoxon Signed Ranks Test). Florescence in situ hybridization analysis also demonstrated that 2F6.3 is a better antibody for the isolation of fetal NRBC in maternal blood than the commercial anti-CD71 antibody.

(J Histochem Cytochem 53:345–350, 2005)

Key Words: erythroblasts • fetal cells • preeclampsia • pregnancy-induced • hypertension • magnetic cell sorting • fluorescence in situ • hybridization


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