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DOI: 10.1369/jhc.4A6520.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (4): 455-465, 2005
Copyright ©The Histochemical Society, Inc.

Chromatoid Bodies : Aggresome-like Characteristics and Degradation Sites for Organelles of Spermiogenic Cells

Celina M. Haraguchi, Tadashi Mabuchi, Shuji Hirata, Tomoko Shoda, Kazuhiko Hoshi, Kenji Akasaki and Sadaki Yokota

Biology Laboratory (CMH,SY), Department of Biochemistry (TM), Department of Obstetrics and Gynecology (SH,TS,KH), University of Yamanashi, Interdisciplinary Graduate School of Medicine and Engineering, Yamanashi, Japan; and Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima, Japan (KA)

Correspondence to: Dr. Sadaki Yokota, Biology Laboratory, University of Yamanashi, Yamanashi, Japan. E-mail: syokota{at}yamanashi.ac.jp

We investigated the localization of several markers for lysosomes and aggresomes in the chromatoid bodies (CBs) by immunoelectron microscopy. We found so-called aggresomal markers such as Hsp70 and ubiquitin in the core of the CBs and vimentin and proteasome subunit around the CBs. Ubiquitin-conjugating enzyme (E2) was also found in the CBs. In tubulovesicular structures surrounding the CBs, lysosomal markers were detected but an endoplasmic reticulum retention signal (KDEL) was not. Moreover, proteins located in each subcellular compartment, including the cytosol, mitochondria, and nucleus, were detected in the CBs. Signals for cytochrome oxidase I (COXI) coded on mitochondrial DNA were also found in the CBs. Quantitative analysis of labeling density showed that all proteins examined were concentrated in the CBs to some extent. These results show that the CBs have some aggresomal features, suggesting that they are not a synthetic site as proposed previously but a degradation site where unnecessary DNA, RNA, and proteins are digested. (J Histochem Cytochem 53:455–465, 2005)

Key Words: chromatoid body • spermiogenesis • degradation • aggresome • immunoelectron microscopy


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