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DOI: 10.1369/jhc.4B6578.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (4): 543-546, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Monoclonal Antibodies to Plant Cell Wall Xylans and Arabinoxylans

Lesley McCartney, Susan E. Marcus and J. Paul Knox

Centre for Plant Sciences, University of Leeds, Leeds, UK

Correspondence to: Dr. Paul Knox, University of Leeds, Centre for Plant Sciences, Faculty of Biological Sciences, Leeds, LS2 9JT, United Kingdom. E-mail: j.p.knox{at}leeds.ac.uk

Two rat monoclonal antibodies have been generated to plant cell wall (1->4)-ß-D-xylans using a penta-1,4-xylanoside–containing neoglycoprotein as an immunogen. The monoclonal antibodies, designated LM10 and LM11, have different specificities to xylans in relation to the substitution of the xylan backbone as indicated by immunodot assays and competitive-inhibition ELISAs. LM10 is specific to unsubstituted or low-substituted xylans, whereas LM11 binds to wheat arabinoxylan in addition to unsubstituted xylans. Immunocytochemical analyses indicated the presence of both epitopes in secondary cell walls of xylem but differences in occurrence in other cell types.

(J Histochem Cytochem 53:543–546, 2005)

Key Words: arabinoxylan • cell wall • glucuronoarabinoxylan • monoclonal antibody • polysaccharide • xylan


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