Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
DOI: 10.1369/jhc.4A6425.2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Puolakkainen, P. A.
Right arrow Articles by Sage, E. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Puolakkainen, P. A.
Right arrow Articles by Sage, E. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 53 (5): 571-581, 2005
Copyright ©The Histochemical Society, Inc.

SPARC-thrombospondin-2-double-null Mice Exhibit Enhanced Cutaneous Wound Healing and Increased Fibrovascular Invasion of Subcutaneous Polyvinyl Alcohol Sponges

Pauli A. Puolakkainen, Amy D. Bradshaw, Rolf A. Brekken, May J. Reed, Themis Kyriakides, Sarah E. Funk, Michel D. Gooden, Robert B. Vernon, Thomas N. Wight, Paul Bornstein and E. Helene Sage

Hope Heart Program, Benaroya Research Institute at Virginia Mason, Seattle, Washington (PAP,ADB,RAB,SEF,MDG,RBV, TNW,EHS); Departments of Medicine (PAP,MJR,PB) and Biochemistry (TK,PB), University of Washington, Seattle, Washington; and Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland (PAP)

Correspondence to: E. Helene Sage, PhD, Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 9th Ave., Seattle, WA 98101. E-mail: hsage{at}benaroyaresearch.org or pauli.puolakkainen{at}hus.fi

Secreted protein acidic and rich in cysteine (SPARC) and thrombospondin-2 (TSP-2) are structurally unrelated matricellular proteins that have important roles in cell–extracellular matrix (ECM) interactions and tissue repair. SPARC-null mice exhibit accelerated wound closure, and TSP-2-null mice show an overall enhancement in wound healing. To assess potential compensation of one protein for the other, we examined cutaneous wound healing and fibrovascular invasion of subcutaneous sponges in SPARC-TSP-2 (ST) double-null and wild-type (WT) mice. Epidermal closure of cutaneous wounds was found to occur significantly faster in ST-double-null mice, compared with WT animals: histological analysis of dermal wound repair revealed significantly more mature phases of healing at 1, 4, 7, 10, and 14 days after wounding, and electron microscopy showed disrupted ECM at 14 days in these mice. ST-double-null dermal fibroblasts displayed accelerated migration, relative to WT fibroblasts, in a wounding assay in vitro, as well as enhanced contraction of native collagen gels. Zymography indicated that fibroblasts from ST-double-null mice also produced higher levels of matrix metalloproteinase (MMP)-2. These data are consistent with the increased fibrovascular invasion of subcutaneous sponge implants seen in the double-null mice. The generally accelerated wound healing of ST-double-null mice reflects that described for the single-null animals. Importantly, the absence of both proteins results in elevated MMP-2 levels. SPARC and TSP-2 therefore perform similar functions in the regulation of cutaneous wound healing, but fine-tuning with respect to ECM production and remodeling could account for the enhanced response seen in ST-double-null mice.

(J Histochem Cytochem 53:571–581, 2005)

Key Words: SPARC • thrombospondin • wound healing • matricellular • extracellular matrix • epidermis • dermis • angiogenesis • collagen


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. S. Weinbaum, T. J. Broekelmann, R. A. Pierce, C. C. Werneck, F. Segade, C. S. Craft, R. H. Knutsen, and R. P. Mecham
Deficiency in Microfibril-associated Glycoprotein-1 Leads to Complex Phenotypes in Multiple Organ Systems
J. Biol. Chem., September 12, 2008; 283(37): 25533 - 25543.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Weaver, G. Workman, and E. H. Sage
The Copper Binding Domain of SPARC Mediates Cell Survival in Vitro via Interaction with Integrin {beta}1 and Activation of Integrin-linked Kinase
J. Biol. Chem., August 15, 2008; 283(33): 22826 - 22837.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
I. Tiala, J. Wakkinen, S. Suomela, P. Puolakkainen, R. Tammi, S. Forsberg, O. Rollman, K. Kainu, B. Rozell, J. Kere, et al.
The PSORS1 locus gene CCHCR1 affects keratinocyte proliferation in transgenic mice
Hum. Mol. Genet., April 1, 2008; 17(7): 1043 - 1051.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
O. J Sansom, F. C Mansergh, M. J Evans, J. A Wilkins, and A. R Clarke
Deficiency of SPARC suppresses intestinal tumorigenesis in APCMin/+ mice
Gut, October 1, 2007; 56(10): 1410 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Sullivan, T. H. Barker, S. E. Funk, A. Karchin, N. S. Seo, M. Hook, J. Sanders, B. Starcher, T. N. Wight, P. Puolakkainen, et al.
Matricellular Hevin Regulates Decorin Production and Collagen Assembly
J. Biol. Chem., September 15, 2006; 281(37): 27621 - 27632.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005