Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

DOI: 10.1369/jhc.4A6498.2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cuesta, N.
Right arrow Articles by Zudaire, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cuesta, N.
Right arrow Articles by Zudaire, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 53 (6): 773-780, 2005
Copyright ©The Histochemical Society, Inc.

Identification of Adrenomedullin in Avian Type II Pneumocytes : Increased Expression after Exposure to Air Pollutants

Natalia Cuesta, Alfredo Martínez, Frank Cuttitta and Enrique Zudaire

Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland (NC), and Cell and Cancer Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland (AM,FC,EZ)

Correspondence to: Natalia Cuesta, PhD, Department of Microbiology and Immunology, School of Medicine, 660 W. Redwood Street, University of Maryland, Baltimore, MD 21201. E-mail: ncuesta{at}som.umaryland.edu

Adrenomedullin (AM) is a potent vasodilator peptide present in the lung of mammals where it is expressed mainly in the columnar epithelium and alveolar macrophages. AM increases the secretion of phosphatidylcholine by type II pneumocytes, which suggests a role as an autocrine modulator of surfactant secretion. In this study we show the expression of an AM-like protein in the lung of the pigeon, Columba livia. Using an antibody against its human ortholog, AM-like immunoreactivity was found to be associated with membranous structures of the multivesicular bodies of type II pneumocytes. We also studied the differential expression of AM-like peptide in the lung of pigeons exposed to polluted city air vs cleaner countryside conditions and found that AM-like expression was higher in city animals. Similar results were obtained in an experimental study in which pigeons were exposed to increasing concentrations of a single pollutant, ozone. Taken together, our findings support the implication of AM in the response of type II pneumocytes to air pollutants.

(J Histochem Cytochem 53:773–780, 2005)

Key Words: adrenomedullin • pigeon • air pollution • lung • surfactant • type II pneumocytes • ozone


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005

 
Purchase HCS Short Course Manual on HCS site