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DOI: 10.1369/jhc.4B6598.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (6): 791-792, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Microdissection-derived Murine Mcb Probes from Somatic Cell Hybrids

Vladimir Trifonov, Constanze Karst, Uwe Claussen, Kristin Mrasek, Susanne Michel, Philip Avner and Thomas Liehr

Institute of Human Genetics and Anthropology, Jena, Germany (VT,CK,UC,KM,SM,TL); Institute of Cytology and Genetics, Novosibirsk, Russia (VT); and Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France (PA)

Correspondence to: Dr. Thomas Liehr, Institut für Humangenetik, Postfach D-07740, Jena, Germany. E-mail: i8lith{at}mti.uni-jena.de

The multicolor-banding (mcb) technique is a fluorescence in situ hybridization (FISH)-banding approach, which is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first three available mcb-probe sets for the Mus musculus chromosomes 3, 6, and 18. In the present work, the creation of the microdissection libraries was done for the first time on mouse/human somatic cell hybrids. During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes. (J Histochem Cytochem 53:791–792, 2005)

Key Words: multicolor banding • murine chromosomes • somatic cell hybrids • fluorescence in situ • hybridization


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