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DOI: 10.1369/jhc.4B6610.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (7): 913-916, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Differential Intracellular Distributions of Inositol Trisphosphate and Ryanodine Receptors Within and Among Hematopoietic Cells

Andrea J. Clark and Howard R. Petty

Departments of Ophthalmology and Visual Sciences (AC,HRP) and Microbiology and Immunology (HRP), The University of Michigan Medical School, Ann Arbor, Michigan

Correspondence to: Dr. Howard R. Petty, Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105. E-mail: hpetty{at}umich.edu

To better understand the mechanism(s) of leukocyte Ca2+ signaling, we have studied the intracellular locations of two Ca2+-mobilizing receptors, the inositol 1,4,5-trisphosphate receptor and ryanodine receptor, by immunofluorescence microscopy. Our results show that localization differs not only between receptor classes within a cell, but among leukocyte types as well. We also illustrate the importance of preserving labile cellular filaments in maintaining cell integrity by fixation with the Safiejko-Mroczka and Bell protocol, because conventional fixation methods distort receptor patterns. We suggest that the observed differences influence intracellular Ca2+ signaling.

(J Histochem Cytochem 53:913–916, 2005)

Key Words: calcium • neutrophil • monocyte • lymphocyte • ryanodine receptor • inositol trisphosphate receptor


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