Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

Originally published as JHC exPRESS on May 27, 2005.
doi:10.1369/jhc.4A6582.2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.4A6582.2005v1
53/8/1023    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raab, M.
Right arrow Articles by Neuhuber, W.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raab, M.
Right arrow Articles by Neuhuber, W.L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 53 (8): 1023-1031, 2005
Copyright ©The Histochemical Society, Inc.

Number and Distribution of Intraganglionic Laminar Endings in the Mouse Esophagus as Demonstrated with Two Different Immunohistochemical Markers

M. Raab and W.L. Neuhuber

Department of Anatomy I, University of Erlangen–Nuremberg, Erlangen, Germany

Correspondence to: M. Raab, Institut für Anatomie, Lehrstuhl I, Krankenhausstr. 9, D-91054 Erlangen, Germany. E-mail: marion.raab{at}anatomie1.med.uni-erlangen.de

Intraganglionic laminar endings (IGLEs) represent the only vagal mechanosensory terminals in the tunica muscularis of the esophagus. Two specific markers for IGLEs were recently described in mouse: the purinergic P2x2 receptor and the vesicular glutamate transporter 2 (VGLUT2). This study aimed at comparing both markers with respect to their suitability for quantitative analysis. We counted IGLEs immunostained for VGLUT2 and P2x2, respectively, and mapped their distribution in esophageal wholemounts of C57Bl/6 mice. Numbers and distribution of IGLEs were compared with those of myenteric ganglia as demonstrated by cuprolinic blue histochemistry. Whereas the distribution of VGLUT2-immunopositive IGLEs closely matched that of myenteric ganglia, P2x2-immunopositive IGLEs were rarely found in upper and middle esophagus but increasingly in its lower parts. P2x2 stained only half the number of IGLEs found with VGLUT2 immunostaining. We also investigated the correlation between anterograde tracing and immunohistochemistry for identifying IGLEs. Confocal microscopy revealed colocalization of all three markers in ~50% of IGLEs. The remaining IGLEs showed only tracer and VGLUT2 labeling but no P2x2 immunoreactivity. Thus, VGLUT2 and P2x2 represent two specific markers for qualitative demonstration of esophageal IGLEs. However, VGLUT2 may be superior to P2x2 as a quantitative marker for IGLEs in the esophagus of C57Bl/6 mice. (J Histochem Cytochem 53:1023–1031, 2005)

Key Words: P2x2 • VGLUT2 • vagal afferents • anterograde tracing • cuprolinic blue • myenteric ganglia • C57Bl/6 mice


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005

 
Purchase HCS Short Course Manual on HCS site