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Originally published as JHC exPRESS on May 27, 2005.
doi:10.1369/jhc.5B6681.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (8): 1037-1040, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Elongation of Peroxisomes as an Indicator for Efficient Dynamin-like Protein 1 Knock Down in Mammalian Cells

Antje Boll and Michael Schrader

Department of Cell Biology and Cell Pathology, University of Marburg, Marburg, Germany

Correspondence to: Michael Schrader, Department of Cell Biology and Cell Pathology, University of Marburg, Robert-Koch Str. 6, D-35037 Marburg, Germany. E-mail: schrader{at}mailer.uni-marburg.de

RNA interference has become a valuable tool to identify and investigate proteins involved in the formation of peroxisomes. We demonstrate that the elongation of peroxisomes serves as an excellent indicator for efficient knock down of dynamin-like protein 1 (DLP1) in mammalian cells. We took advantage of the silencing-dependent morphological changes of peroxisomes to compare different transfection methods and show that a single transfection of DLP1 siRNA by electroporation is sufficient to effectively silence DLP1. We present a fast, easy, and convenient protocol for efficient gene silencing in a large number of cells, which can be used for quantitative and biochemical studies.

(J Histochem Cytochem 53:1037–1040, 2005)

Key Words: peroxisomes • dynamin-like protein 1 • siRNA • electroporation • oligofectamine


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H. K. Delille and M. Schrader
Targeting of hFis1 to Peroxisomes Is Mediated by Pex19p
J. Biol. Chem., November 7, 2008; 283(45): 31107 - 31115.
[Abstract] [Full Text] [PDF]



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