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Originally published as JHC exPRESS on June 13, 2005.
doi:10.1369/jhc.5B6691.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (9): 1167-1170, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Protein-embedding Technique : A Potential Approach to Standardization of Immunohistochemistry for Formalin-fixed, Paraffin-embedded Tissue Sections

Shan-Rong Shi, Cheng Liu, Jeanette Perez and Clive R. Taylor

Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California

Correspondence to: Clive R. Taylor, MD, PhD, Department of Pathology, University of Southern California Keck School of Medicine, HMR 204, 2011 Zonal Avenue, Los Angeles, CA 90033. E-mail: taylor{at}pathfinder.hsc.usc.edu

A serial study was performed to develop a protein-embedding technique for standardization of immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. A protein carrier matrix must have two phases, a liquid phase to allow uniform mixing of a protein and a solid phase forming a ‘block’ that can be fixed and processed in the same manner as human tissue. This standardized protein block would serve as a source of thin sections for control of IHC and therefore must also withstand the boiling conditions of antigen retrieval (AR). After multiple experiments, a method was developed utilizing polymer microsphere (beads) as a support medium for protein. The method showed particular promise for quantitative IHC.

(J Histochem Cytochem 53:1167–1170, 2005)

Key Words: immunohistochemistry • antigen retrieval • standardization • protein-embedding-technique • beads


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[Abstract] [Full Text] [PDF]



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