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Originally published as JHC exPRESS on May 27, 2005.
doi:10.1369/jhc.5B6708.2005
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Journal of Histochemistry and Cytochemistry
Volume 53 (9): 1177-1180, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Establishment of Stable GH3 Cell Line Expressing Enhanced Yellow Fluorescein Protein–Growth Hormone Fusion Protein

Akira Matsuno, Akiko Mizutani, Johbu Itoh, Susumu Takekoshi, Tadashi Nagashima, Hiroko Okinaga, Koji Takano and R. Yoshiyuki Osamura

Department of Neurosurgery, Teikyo University Ichihara Hospital, Ichihara City, Chiba, Japan (AMa,TN); Basic Medical Science and Molecular Medicine (AMi), Teaching and Research Support Center (JI), and Department of Pathology (ST,RYO), Tokai University School of Medicine, Boseidai, Isehara City, Kanagawa, Japan; and Division of Endocrinology, Department of Internal Medicine, University of Tokyo Hospital, Bunkyo-ku, Tokyo, Japan (HO,KT)

Correspondence to: Akira Matsuno, MD, PhD, Department of Neurosurgery, Teikyo University Ichihara Hospital, 3426-3 Anesaki, Ichihara City, Chiba 299-0111, Japan. E-mail: akirakun{at}med.teikyo-u.ac.jp

To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH. (J Histochem Cytochem 53:1177–1180, 2005)

Key Words: EYFP • GH • GH3 cell


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