Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

Originally published as JHC exPRESS on May 27, 2005.
doi:10.1369/jhc.5B6708.2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.5B6708.2005v1
53/9/1177    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsuno, A.
Right arrow Articles by Osamura, R. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsuno, A.
Right arrow Articles by Osamura, R. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 53 (9): 1177-1180, 2005
Copyright ©The Histochemical Society, Inc.

BRIEF REPORT

Establishment of Stable GH3 Cell Line Expressing Enhanced Yellow Fluorescein Protein–Growth Hormone Fusion Protein

Akira Matsuno, Akiko Mizutani, Johbu Itoh, Susumu Takekoshi, Tadashi Nagashima, Hiroko Okinaga, Koji Takano and R. Yoshiyuki Osamura

Department of Neurosurgery, Teikyo University Ichihara Hospital, Ichihara City, Chiba, Japan (AMa,TN); Basic Medical Science and Molecular Medicine (AMi), Teaching and Research Support Center (JI), and Department of Pathology (ST,RYO), Tokai University School of Medicine, Boseidai, Isehara City, Kanagawa, Japan; and Division of Endocrinology, Department of Internal Medicine, University of Tokyo Hospital, Bunkyo-ku, Tokyo, Japan (HO,KT)

Correspondence to: Akira Matsuno, MD, PhD, Department of Neurosurgery, Teikyo University Ichihara Hospital, 3426-3 Anesaki, Ichihara City, Chiba 299-0111, Japan. E-mail: akirakun{at}med.teikyo-u.ac.jp

To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH. (J Histochem Cytochem 53:1177–1180, 2005)

Key Words: EYFP • GH • GH3 cell


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. Takaya, T. Kamio, M. Masuda, N. Mochizuki, H. Sawa, M. Sato, K. Nagashima, A. Mizutani, A. Matsuno, E. Kiyokawa, et al.
R-Ras Regulates Exocytosis by Rgl2/Rlf-mediated Activation of RalA on Endosomes
Mol. Biol. Cell, May 1, 2007; 18(5): 1850 - 1860.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2005

 
Purchase HCS Short Course Manual on HCS site