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Expression of Prostasin and Protease Nexin-1 in Rhesus Monkey (Macaca mulatta) Endometrium and Placenta During Early Pregnancy

Hai-Yan Lin, Heng Zhang, Qing Yang, Hong-Xing Wang, Hong-Mei Wang, Karl X. Chai, Li-Mei Chen and Cheng Zhu

State Key Laboratory of Reproductive Biology, Institute of Zoology (H-YL,HZ,QY,H-XW,H-MW,CZ) and Graduate School (HZ,QY,H-XW), Chinese Academy of Sciences, Beijing, People's Republic of China, and Department of Molecular Biology and Microbiology (KXC,L-MC) and Biomolecular Science Center (KXC), University of Central Florida, Orlando, Florida

Correspondence to: Prof. Cheng Zhu, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Lu, Haidian District, Beijing 100080, China. E-mail: zhuc{at}ioz.ac.cn Co-corresponding author: Dr. Li-Mei Chen. E-mail: zhuc{at}ioz.ac.cn

Serine proteases have been documented to play key roles in uterine matrix turnover and trophoblastic invasion during implantation. Roles of prostasin serine protease in these processes, however, are currently unclear. The present study was first conducted to investigate the colocalization of prostasin and its cognate serpin, protease nexin-1 (PN-1), in rhesus monkey endometrium and placenta on days 12, 18, and 26 of pregnancy by using in situ hybridization (ISH) and immunohistochemistry. With ISH, expression of prostasin mRNA was intensely localized in the glandular epithelium on days 12 and 18 and in the placental villi, trophoblastic column, trophoblastic shell, and fetal–maternal border on days 18 and 26. With the progress of pregnancy, expression level in the glandular epithelium was significantly decreased, and the accumulation in the placental compartments was further increased. In addition, the stroma and arterioles exhibited modest levels of prostasin signals. However, expression level of PN-1 in these compartments on adjacent sections in the three stages of early pregnancy was weak or below the level of detection. Prostasin protein expression in the endometrium was found to be consistent with the distribution patterns revealed in the ISH experiments. It may be suggested from these results that prostasin is involved in endometrial epithelial morphology establishment, tissue remodeling, and trophoblastic invasion during early pregnancy. The cognate serpin PN-1 was not coordinately expressed along with prostasin, creating a tissue environment favorable for proteolytic activities of prostasin during early pregnancy events. (J Histochem Cytochem 54:1139–1147, 2006)

Key Words: prostasin • protease nexin-1 • endometrium • placenta • rhesus monkey • pregnancy

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				H. Zhang, H.-Y. Lin, Q. Yang, H.-X. Wang, K. X. Chai, L.-M. Chen, and C. Zhu Expression of Prostasin Serine Protease and Protease Nexin-1 (PN-1) in Rhesus Monkey Ovary During Menstrual Cycle and Early Pregnancy J. Histochem. Cytochem., December 1, 2007; 55(12): 1237 - 1244. [Abstract] [Full Text] [PDF]

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