This Article Full Text Full Text (PDF) All Versions of this Article: jhc.5A6805.2005v1 54/4/457    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reimers, D. Articles by Bazán, E. Search for Related Content PubMed PubMed Citation Articles by Reimers, D. Articles by Bazán, E. Social Bookmarking                      What's this?

Intrastriatal Infusion of Liver Growth Factor Stimulates Dopamine Terminal Sprouting and Partially Restores Motor Function in 6-Hydroxydopamine-lesioned Rats

Diana Reimers¹, Antonio S. Herranz¹, Juan José Díaz-Gil, M^a Val T. Lobo, Carlos L. Paíno, Raquel Alonso, M^a José Asensio, Rafael Gonzalo-Gobernado and Eulalia Bazán

Servicio de Neurobiología, Hospital Ramón y Cajal, Madrid, Spain (DR,ASH,MVTL,CLP,RA,MJA,RG-G,EB); Servicio de Bioquímica Experimental, Hospital Puerta de Hierro, Madrid, Spain (JJD-G); and Departamento de Biología Celular y Genética, Universidad de Alcalá, Madrid, Spain (MVTL)

Correspondence to: Dr. Eulalia Bazán, Servicio de Neurobiología–Investigacíon, Hospital Ramón y Cajal, Carretera de Colmenar Km. 9.1, 28034 Madrid, España. E-mail: eulalia.bazan{at}hrc.es

Liver growth factor (LGF) is a mitogen for liver cells that shows biological activity in extrahepatic sites and may be useful for neuroregenerative therapies. The aim of this work was to investigate the effects of the intrastriatal (IS) infusion of LGF in the 6-hydroxydopamine rat model of Parkinson's disease. Tyrosine hydroxylase-positive innervation was significantly increased in the dopamine-denervated striatum of rats receiving intrastriatal LGF infusions (160 ng/day/rat x15 days) as compared with a vehicle-infused group. There was no evidence of dopaminergic neurogenesis in the striatum or substantia nigra in any experimental group at the times studied. However, in those animals undergoing IS-LGF infusion for 48 hr, we found a significant increase in both microglial proliferation and in the number of microglial cells that acquired the ameboid morphology. This is characteristic of activated microglia/macrophages that has been reported to play an important role in dopamine terminal sprouting. In summary, our study shows that IS infusion of LGF stimulates the outgrowth of tyrosine hydroxylase-positive terminals in the striatum of 6-hydroxydopamine-treated rats. As apomorphine-induced rotational behavior was also reduced in these animals, we propose LGF as a novel factor that, when delivered to the striatum, may be useful in the treatment of Parkinson's disease. (J Histochem Cytochem 54:457–465, 2006)

Key Words: neuroregeneration • neurogenesis • trophic factors • dopamine • Parkinson's disease • liver growth factor • microglia

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Gonzalo-Gobernado, D. Reimers, A. S. Herranz, J. J. Diaz-Gil, C. Osuna, M. J. Asensio, S. Baena, M. Rodriguez-Serrano, and E. Bazan Mobilization of Neural Stem Cells and Generation of New Neurons in 6-OHDA-lesioned Rats by Intracerebroventricular Infusion of Liver Growth Factor J. Histochem. Cytochem., May 1, 2009; 57(5): 491 - 502. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006