This Article Full Text Full Text (PDF) Supplemental Data for this article All Versions of this Article: jhc.5A6824.2006v1 54/6/673    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rippstein, P. Articles by O'Brien, E. R. Search for Related Content PubMed PubMed Citation Articles by Rippstein, P. Articles by O'Brien, E. R. Social Bookmarking                      What's this?

Comparison of Processing and Sectioning Methodologies for Arteries Containing Metallic Stents

Peter Rippstein, Melanie K. Black, Marie Boivin, John P. Veinot, Xiaoli Ma, Yong-Xiang Chen, Paul Human, Peter Zilla and Edward R. O'Brien

Core Pathology Laboratory (PR) and Division of Cardiology, Vascular Biology Laboratory (XM,Y-XC,ERO), University of Ottawa Heart Institute, Ottawa, Ontario, Canada; Department of Laboratory Medicine, The Ottawa Hospital, Ottawa, Ontario, Canada (MB,JPV); and Christian Barnard Division of Cardiothoracic Surgery, Cape Heart Center, University of Cape Town, Cape Town, South Africa (MKB,PH,PZ)

Correspondence to: Peter Rippstein, ART, MLT, Core Pathology Laboratory, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H2102, Ottawa, Ontario, Canada K1Y 4W7. E-mail: prippstein{at}ottawaheart.ca

The histological study of arteries with implanted metallic scaffolding devices, known as stents, remains a technical challenge. Given that the arterial response to stent implantation can sometimes lead to adverse outcomes, including the re-accumulation of tissue mass within the stent (or in-stent restenosis), overcoming these technical challenges is a priority for the advancement of research and development in this important clinical field. Essentially, the task is to section the stent–tissue interface with the least amount of disruption of tissue and cellular morphology. Although many methacrylate resin methodologies are successfully applied toward the study of endovascular stents by a variety of research laboratories, the exact formulations, as well as subsequent processing and sectioning methodology, remain largely coveted. In this paper, we describe in detail a methyl methacrylate resin–embedding methodology that can successfully be applied to tungsten carbide blade, as well as saw and grinding sectioning methods and transmission electron microscopy. In addition, we present a comparison of the two sectioning methodologies in terms of their effectiveness with regard to morphological, histochemical, and immunohistochemical analyses. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 54:673–681, 2006)

Key Words: stent • artery • restenosis • methyl methacrylate • resin • immunohistochemistry

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Zilla, P. Human, M. Wolf, W. Lichtenberg, N. Rafiee, D. Bezuidenhout, N. Samodien, C. Schmidt, and T. Franz Constrictive external nitinol meshes inhibit vein graft intimal hyperplasia in nonhuman primates J. Thorac. Cardiovasc. Surg., September 1, 2008; 136(3): 717 - 725. [Abstract] [Full Text] [PDF]

				S. Rousselle and J. Wicks Preparation of Medical Devices for Evaluation Toxicol Pathol, January 1, 2008; 36(1): 81 - 84. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006