This Article Full Text Full Text (PDF) All Versions of this Article: jhc.5A6816.2006v1 54/7/745    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hallikas, O. K. Articles by Schröder, J. Search for Related Content PubMed PubMed Citation Articles by Hallikas, O. K. Articles by Schröder, J. Social Bookmarking                      What's this?

Identification of Antibodies against HAI-1 and Integrin 6ß4 as Immunohistochemical Markers of Human Villous Cytotrophoblast

Outi K. Hallikas, Johanna M. Aaltonen, Harriet von Koskull, Lars-Axel Lindberg, Leena Valmu, Nisse Kalkkinen, Torsten Wahlström, Hiroaki Kataoka, Leif Andersson, Dan Lindholm and Jim Schröder

Department of Biological and Environmental Sciences (OKH,JS), Department of Basic Veterinary Sciences (L-AL), Institute of Biotechnology, Protein Chemistry Laboratory (LV,NK), Haartman Institute (TW), and Department of Pathology (LA), University of Helsinki, Helsinki, Finland; Minerva Medical Research Institute, Biomedicum Helsinki, Helsinki, Finland (JMA,DL); Department of Obstetrics and Gynecology/Genetics, Helsinki University Hospital, Helsinki, Finland (HvK); and Second Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan (HK)

Correspondence to: Jim Schröder, Department of Biological and Environmental Sciences, Division of Genetics, Viikinkaari 5, Biocenter 2, PO Box 56, FIN-00014, University of Helsinki, Helsinki, Finland. E-mail: Jim.Schroder{at}helsinki.fi

Syncytiotrophoblast and invasive extravillous trophoblast arise from a common stem cell, namely villous cytotrophoblast, but have very different characteristics. The study of the differentiation process relies on the availability of suitable markers for these different cell types of developing placenta. In this work, we have produced monoclonal antibodies that are specific to human villous cytotrophoblast. Monoclonal antibody (MAb) MG2 was specific to villous cytotrophoblast across gestation, and recognizes hepatocyte growth factor activator inhibitor type 1. MAb MD10 stained villous cytotrophoblast across gestation and also some endothelial cells, particularly in the second or third trimester. MAb MD10 recognizes human integrin 6ß4. As a test for specificity, the novel MAbs were also used for staining of frozen tissue from human colon carcinoma. The results show that the two antibodies can be used as tools to study human villous cytotrophoblasts and also human tumors. The MG2 antibody seems most specific and promising for the study of various aspects of human villous cytotrophoblast. (J Histochem Cytochem 54:745–752, 2006)

Key Words: placenta • villous cytotrophoblast • monoclonal antibodies

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006