This Article Full Text Full Text (PDF) All Versions of this Article: jhc.6A6922.2006v1 54/7/829    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Webster, P. Articles by St. Geme, J. W. Search for Related Content PubMed PubMed Citation Articles by Webster, P. Articles by St. Geme, J. W., III Social Bookmarking                      What's this?

Distribution of Bacterial Proteins in Biofilms Formed by Non-typeable Haemophilus influenzae

Paul Webster, Siva Wu, Gerardo Gomez, Michael Apicella, Andrew G. Plaut and Joseph W. St. Geme, III

Ahmanson Advanced Electron Microscopy and Imaging Center, House Ear Institute, Los Angeles, California (PW,SW,GG); Department of Microbiology, University of Iowa, Iowa City, Iowa (MA); Gastroenterology Research, Tufts-New England Medical Center, Boston, Massachusetts (AGP); and Department of Pediatrics, Duke University Medical Center, Durham, North Carolina (JWSG)

Correspondence to: Paul Webster, PhD, Director, Ahmanson Advanced Electron Microscopy and Imaging Center, House Ear Institute, 2100 West Third Street, Los Angeles, CA 90057. E-mail: pwebster{at}hei.org

The ability to preserve the fragile ultrastructural organization of bacterial biofilms using cryo-preparation methods for electron microscopy has enabled us to probe sections through non-typeable Haemophilus influenzae (NTHi) biofilms and determine the localization of NTHi-specific lipooligosaccharide (LOS) and proteins within these structures. Some of the proteins we examined are currently being considered as candidates for vaccine development, so it is important that their distribution and accessibility within the biofilms formed by NTHi be determined. We have localized LOS to the extracellular matrix (ECM) of the biofilm and the P6 outer membrane protein to the membrane of what appear to be viable bacteria within the biofilm. The Hap and HWM1/HMW2 adhesive proteins were associated with bacteria within the biofilm and were present in the biofilm ECM. The IgA1 protease is a secreted protein that was also associated with NTHi in the biofilm and was in the ECM, but was more concentrated in the top region of the biofilm, suggesting a role in protecting biofilm bacteria from antibody attack. (J Histochem Cytochem 54:829–842, 2006)

Key Words: OMP P6 • HMW proteins • Hap protein • IgA1 protease • electron microscopy • extracellular matrix • immunocytochemistry • stereology

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. T. Arce, R. Carlson, J. Monds, R. Veeh, F. Z. Hu, P. S. Stewart, R. Lal, G. D. Ehrlich, and R. Avci Nanoscale Structural and Mechanical Properties of Nontypeable Haemophilus influenzae Biofilms J. Bacteriol., April 15, 2009; 191(8): 2512 - 2520. [Abstract] [Full Text] [PDF]

				H. Palsdottir, J. P. Remis, C. Schaudinn, E. O'Toole, R. Lux, W. Shi, K. L. McDonald, J. W. Costerton, and M. Auer Three-Dimensional Macromolecular Organization of Cryofixed Myxococcus xanthus Biofilms as Revealed by Electron Microscopic Tomography J. Bacteriol., April 1, 2009; 191(7): 2077 - 2082. [Abstract] [Full Text] [PDF]

				R. A. Brady, J. G. Leid, J. Kofonow, J. W. Costerton, and M. E. Shirtliff Immunoglobulins to Surface-Associated Biofilm Immunogens Provide a Novel Means of Visualization of Methicillin-Resistant Staphylococcus aureus Biofilms Appl. Envir. Microbiol., October 15, 2007; 73(20): 6612 - 6619. [Abstract] [Full Text] [PDF]

				D. Mukundan, Z. Ecevit, M. Patel, C. F. Marrs, and J. R. Gilsdorf Pharyngeal Colonization Dynamics of Haemophilus influenzae and Haemophilus haemolyticus in Healthy Adult Carriers J. Clin. Microbiol., October 1, 2007; 45(10): 3207 - 3217. [Abstract] [Full Text] [PDF]

				D. A. Daines, M. H. Wu, and S. Y. Yuan VapC-1 of Nontypeable Haemophilus influenzae Is a Ribonuclease J. Bacteriol., July 15, 2007; 189(14): 5041 - 5048. [Abstract] [Full Text] [PDF]

				J. A. Jurcisek and L. O. Bakaletz Biofilms Formed by Nontypeable Haemophilus influenzae In Vivo Contain both Double-Stranded DNA and Type IV Pilin Protein J. Bacteriol., May 15, 2007; 189(10): 3868 - 3875. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006