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Originally published as JHC exPRESS on March 20, 2006.
doi:10.1369/jhc.5C6895.2006
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Journal of Histochemistry and Cytochemistry
Volume 54 (8): 849-853, 2006
Copyright ©The Histochemical Society, Inc.

RAPID COMMUNICATION

Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin

Anna A. Bencsik, Anthony W. Coleman, Sabine O.S. Debeer, Hervé Perron and Aly Moussa

Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, France (AAB,SOSD,AM); Institut de Biologie et de Chimie des Protéines, University Lyon 1, Lyon, France (AWC); and BioMérieux, R&D Immunoassay and Proteomics Department, Marcy l'Etoile, France (HP)

Correspondence to: Dr. Anna Bencsik, Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC; 31 avenue Tony Garnier 69364, Lyon, Cedex 07-France. E-mail: a.bencsik{at}lyon.afssa.fr

Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt–Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies. (J Histochem Cytochem 54:849–853, 2006)

Key Words: amplification • bovine spongiform encephalopathy • diagnosis • immunohistochemistry • prion • scrapie • streptomycin


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