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Originally published as JHC exPRESS on March 3, 2006.
doi:10.1369/jhc.5A6792.2006
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Journal of Histochemistry and Cytochemistry
Volume 54 (8): 877-888, 2006
Copyright ©The Histochemical Society, Inc.

The Secretory Granule Protein Syncollin Localizes to HL-60 Cells and Neutrophils

Jan-Philipp Bach, Heike Borta, Waltraud Ackermann, Floriane Faust, Oliver Borchers and Michael Schrader

Department of Cell Biology and Cell Pathology, University of Marburg, Marburg, Germany

Correspondence to: Michael Schrader, Department of Cell Biology and Cell Pathology, University of Marburg, Robert-Koch Str. 6, D-35037 Marburg, Germany. E-mail: schrader{at}mailer.uni-marburg.de

The secretory granule protein syncollin was first identified in the exocrine pancreas where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In this study we provide first morphological and biochemical evidence that, in addition to its pancreatic localization, syncollin is also present in neutrophilic granulocytes of rat and human origin. By immunohistological studies, syncollin was detected in neutrophilic granulocytes of the spleen. Furthermore, syncollin is expressed by the promyelocytic HL-60 cells, where it is stored in azurophilic granules and in a vesicular compartment. These findings were confirmed by fractionation experiments and immunoelectron microscopy. Treatment with a phorbol ester triggered the release of syncollin indicating that in HL-60 cells it is a secretory protein that can be mobilized upon stimulation. A putative role for syncollin in host defense is discussed. (J Histochem Cytochem 54:877–888, 2006)

Key Words: pancreas • granule biogenesis • neutrophil • secretion • HL-60 • antimicrobial


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