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Expression of Bone Morphogenic Proteins and Receptors at the Injured Growth Plate Cartilage in Young Rats

Thai Q. Ngo, Michaela A. Scherer, Fiona H. Zhou, Bruce K. Foster and Cory J. Xian

Department of Orthopaedic Surgery, Women's and Children's Hospital, North Adelaide, Australia (TQN,MAS,FHZ,BKF,CJX); Department of Pharmaceutical Biotechnology, University of South Australia, Adelaide, Australia (TQN,CJX); and Department of Paediatrics, University of Adelaide, Adelaide, Australia (FHZ,BKF,CJX)

Correspondence to: Dr. Cory J. Xian, Department of Orthopaedic Surgery, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006 Australia. E-mail: cory.xian{at}adelaide.edu.au

The injured growth plate cartilage is often repaired by bony tissue, resulting in impaired bone growth in children. Bone morphogenic proteins (BMPs) are important for bone fracture repair, and as a step to characterize potential involvement of BMPs in bony repair of injured growth plate, expression of BMPs and receptors (BMP-R) was examined by quantitative RT-PCR and immunohistochemistry in rat injured tibial growth plate. During the inflammatory response on day 1, slightly increased expression of BMP-3, BMP-4, BMP-R1a, and BMP-R2 was observed, with immunostaining seen among inflammatory cells at the injury site. During mesenchymal infiltration and osteogenic responses on days 3–14, moderately increased expression of BMP-2, -3, -4, -7, and BMP-R1a was found, with immunostaining observed among infiltrated mesenchymal cells and differentiated osteoblasts lining bony trabeculae. During maturation phase on days 14–25, only BMP-7 was seen upregulated slightly and was localized in osteoblasts and marrow cells at the injury site. The temporospatial expression of BMPs and receptors at the injured growth plate suggests potential involvement of BMP-3 and -4 in regulating the inflammatory response or as its mediators in modulating downstream events, and BMP-2, -3, -4, and -7 in the fibrogenic and osteogenic responses, and BMP-7 in bone remodeling at the injured growth plate. (J Histochem Cytochem 54:945–954, 2006)

Key Words: growth plate • injury and repair • injury responses • bone bridge • BMP • expression

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