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Differential Expression of Cell Surface Markers by Ovine Respiratory Tract Dendritic Cells

Tom N. McNeilly, Jeremy K. Brown and Gordon Harkiss

Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Easter Bush, Midlothian, United Kingdom

Correspondence and present address: Tom N. McNeilly, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, UK. E-mail: Tom.McNeilly{at}Moredun.ac.uk

Dendritic cells (DCs) are key antigen-presenting cells central to the induction of primary immune responses. Despite the prevalence of respiratory disease in sheep and the increasing use of the ovine lung as a model for human disease, ovine respiratory tract DCs (RTDCs) have not yet been characterized. Using single and double immunocytochemical staining, expression of a number of potential DC markers (MHC class II, CD1b, SIRP, and CD205) by ovine RTDC populations has been determined. MHC class II staining revealed widespread populations of DCs either adjacent to respiratory airway epithelium or within the lung parenchyma. CD1b was expressed by a small subpopulation of both airway and parenchymal RTDCs. Expression of SIRP was limited to a small subpopulation of airway RTDCs but was absent from the lung parenchyma. CD205 was widely expressed by airway RTDCs but expressed only by a small subpopulation of parenchymal RTDCs. In addition, the majority (87%) of parenchymal CD205⁺ cells exhibited a non-DC-like morphology and did not express MHC class II, suggesting that these single CD205⁺ cells were not DCs. Phenotypic differences between airway and parenchymal RTDCs may be related to functional differences between the two populations. (J Histochem Cytochem 54:1021–1030, 2006)

Key Words: ovine • respiratory tract • dendritic cells • CD1b • SIRP • CD205

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