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Originally published as JHC exPRESS on April 3, 2006.
doi:10.1369/jhc.5A6733.2006
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Journal of Histochemistry and Cytochemistry
Volume 54 (9): 981-990, 2006
Copyright ©The Histochemical Society, Inc.

Immunohistochemical Study of Cell Proliferation, Bcl-2, p53, and Caspase-3 Expression on Preneoplastic Changes Induced by Cadmium and Zinc Chloride in the Ventral Rat Prostate

Riánsares Arriazu, José M. Pozuelo, Nuno Henriques-Gil, Teresa Perucho, Rocío Martín, Rosario Rodríguez and Luis Santamaría

Cell Biology and Histology Laboratory (RA,JMP,RR) and Genetics Laboratory (NH-G,TP), San Pablo–CEU University, Madrid, Spain; Service of Pathology, Hospital N. Sra. de Sonsoles, Ávila, Spain (RM); and Department of Morphology, School of Medicine, Autonomous University of Madrid, Madrid, Spain (LS)

Correspondence to: L. Santamaría, Department of Morphology, School of Medici ne, Autonomous University, C/Arzobispo Morcillo, 2, E-28029 Madrid, Spain. E-mail: luis.santamaria{at}uam.es

This work was directed to evaluate immunoexpression of markers for apoptosis, resistance to apoptosis, and cell proliferation, as well as estimates of nuclear size in ventral prostate of rats treated with cadmium chloride and cadmium + zinc chloride because a possible protective effect of zinc has been postulated. The following variables were studied: volume fraction (VF) of Bcl-2 immunostaining, percentage of cells immunoreactive to proliferating cell nuclear antigen (LIPCNA) and p53 (LIp53), numerical density of caspase-3 immunoreactive cells (NV caspase-3), and estimates of volume-weighted mean nuclear volume ({upsilon}V). The LIPCNA and VF of Bcl-2 were significantly increased in the treated animals. The dysplasias (independent of their origin) showed a significant increase of the LIp53, NV caspase-3, and {upsilon}V in comparison with normal acini from treated and control animals. It can be concluded that cell proliferation is enhanced in long-term cadmium-exposed rats, and exposure to zinc combined with cadmium had no effect on any of the variables studied when comparing with normal acini. The increase of nuclear {upsilon}V could indicate a more aggressive behavior for pretumoral lesions. (J Histochem Cytochem 54:981–990, 2006)

Key Words: rat prostate • cadmium • zinc • dysplasia • immunohistochemistry


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