This Article Full Text Full Text (PDF) All Versions of this Article: jhc.6A6952.2006v1 55/1/1    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhang, H.-T. Articles by Wang, T. T.-H. Search for Related Content PubMed PubMed Citation Articles by Zhang, H.-T. Articles by Wang, T. T.-H. Social Bookmarking                         What's this?

Immunohistochemical Distribution of NGF, BDNF, NT-3, and NT-4 in Adult Rhesus Monkey Brains

Hong-Tian Zhang, Li-Yan Li, Xiao-Li Zou, Xin-Bo Song, Yan-Li Hu, Zhong-Tang Feng and Tim Ting-Hua Wang

Institute of Neuroscience, Kunming Medical College, Kunming, China (H-TZ,L-YL,X-BS,Y-LH,Z-TF,TT-HW), and Department of Histology, Embryology and Neurobiology, College of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China (X-LZ,TT-HW)

Correspondence to: Professor Tim Ting-Hua Wang, Institute for Research on Neuroscience, Kunming Medical College, No. 191 Renming West Road, Kunming 650031, China. E-mail: tinghua_neuron{at}263.net

Immunohistochemical distribution and cellular localization of neurotrophins was investigated in adult monkey brains using antisera against nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). Western blot analysis showed that each antibody specifically recognized appropriate bands of 14.7 kDa, 14.2 kDa, 13.6 kDa, and 14.5 kDa, for NGF, BDNF, NT-3, and NT-4, respectively. These positions coincided with the molecular masses of the neurotrophins studied. Furthermore, sections exposed to primary antiserum preadsorbed with full-length NGF, BDNF, NT-3, and NT-4 exhibited no detectable immunoreactivity, demonstrating specificities of the antibodies against the tissues prepared from rhesus monkeys. The study provided a systematic report on the distribution of NGF, BDNF, NT-3, and NT-4 in the monkey brain. Varying intensity of immunostaining was observed in the somata and processes of a wide variety of neurons and glial cells in the cerebrum, cerebellum, hippocampus, and other regions of the brain. Neurons in some regions such as the cerebral cortex and the hippocampus, which stained for neurotrophins, also expressed neurotrophic factor mRNA. In some other brain regions, there was discrepancy of protein distribution and mRNA expression reported previously, indicating a retrograde or anterograde action mode of neurotrophins. Results of this study provide a morphological basis for the elucidation of the roles of NGF, BDNF, NT-3, and NT-4 in adult primate brains. (J Histochem Cytochem 55:1–19, 2007)

Key Words: nerve growth factor • brain-derived neurotrophic factor • neurotrophin-3 • neurotrophin-4 • immunohistochemistry • distribution • monkey

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007