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Spatial and Temporal Control of Laminin-332 (5) and -511 (10) Expression During Induction of Anagen Hair Growth

Koji Sugawara, Daisuke Tsuruta, Hiromi Kobayashi, Kazuo Ikeda, Susan B. Hopkinson, Jonathan C.R. Jones and Masamitsu Ishii

Department of Dermatology (KS,DT,HK,MI) and Department of Anatomy (KI), Osaka City University Graduate School of Medicine, Osaka, Japan, and Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois (SBH,JCRJ)

Correspondence to: Daisuke Tsuruta, MD, PhD, Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan. E-mail: dtsuruta{at}med.osaka-cu.ac.jp

Basement membrane plays important roles in hair growth. We characterized changes in laminin isoform expression during hair cycling. At the mRNA level, laminin-511 (10) expression underwent a steady increase during anagen stages. In contrast, laminin-332 (5) expression was initially upregulated in outer root sheath (ORS) keratinocytes at anagen II and then transiently downregulated. Laminin-332 significantly increased coincident with the signal in inner root sheath and hair matrix cells after anagen IV. Levels of laminin-332 proteins were also upregulated at late anagen I–III but dropped after anagen IV. This decrease coincided with increased levels of mRNA encoding the two proteases, membrane type 1 metalloproteinase and bone morphogenetic protein 1, involved in laminin-332 processing. Immunohistochemistry demonstrated that laminin-332 and 6ß4 integrin were well colocalized, but their signals were remarkably decreased in the lower half of follicles after anagen VI. Consistent with these data, ultrastructurally mature hemidesmosomes were seen in ORS keratinocytes at anagen II, whereas at anagen VI, only fragmental hemidesmosomes were present. In hair follicle culture, laminin-511 (10)/521 (11)-rich human placental laminin enhanced hair growth, whereas recombinant laminin-332 antagonized hair growth induced by laminin-511. Our results indicate a positive role for laminin-511 and a negative role for laminin-332 on hair growth. (J Histochem Cytochem 55:43–55, 2007)

Key Words: laminin • hemidesmosome • integrin • protease • hair cycle

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