Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
Originally published as JHC exPRESS on September 18, 2006.
doi:10.1369/jhc.6A6962.2006
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data for this article
Right arrow All Versions of this Article:
jhc.6A6962.2006v1
55/1/71    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stahlman, M. T.
Right arrow Articles by Whitsett, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stahlman, M. T.
Right arrow Articles by Whitsett, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 55 (1): 71-83, 2007
Copyright ©The Histochemical Society, Inc.

Expression of ABCA3 in Developing Lung and Other Tissues

Mildred T. Stahlman, Valérie Besnard, Susan E. Wert, Timothy E. Weaver, Sharon Dingle, Yan Xu, Kara von Zychlin, Sandra J. Olson and Jeffrey A. Whitsett

Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center and The University of Cincinnati College of Medicine, Cincinnati, Ohio (TEW,SEW,VB,YX,KVZ,SD,JAW), and Divisions of Neonatology (MTS) and Pathology (SJO), Vanderbilt University School of Medicine, Nashville, Tennessee

Correspondence to: Jeffrey A. Whitsett, MD, Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039. E-mail: jeff.whitsett{at}cchmc.org

ABCA3 is a member of the ATP-binding cassette (ABC) family of transport proteins and is required for perinatal respiratory adaptation. Monoclonal and polyclonal antibodies were generated against a recombinant human ABCA3 peptide and used to assess its expression in the developing lung and adult tissues. Immunostaining for ABCA3 was detected at highest levels in type II epithelial cells of the lung but was also noted in other organs including liver, stomach, kidney, adrenal, pancreas, trachea, and brain. In the fetal lung, ABCA3 staining and mRNA increased prior to birth. Like other surfactant protein genes, ABCA3 expression was induced by thyroid transcription factor-1 in vitro. ABCA3 was coexpressed with SP-B and proSP-C in type II epithelial cells. ABCA3 staining was detected surrounding large, intracellular organelles consistent with its association with lamellar bodies. In the human fetal lung, ABCA3 staining was not detected prior to 22–23 weeks of gestation, except in the presence of pulmonary inflammation. ABCA3 was detected in type II epithelial cells of the human lung from 28 weeks of gestation and thereafter. Postnatally, intense ABCA3 staining was observed in hyperplastic epithelial cells relining injured airways in infants with chronic lung disease. Localization and regulation of ABCA3 in the respiratory epithelium is consistent with its proposed role in surfactant homeostasis. The role of ABCA3 in extrapulmonary tissues and organs remains to be elucidated. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 55:71–83, 2007)

Key Words: ABC transporters • pulmonary surfactant • lamellar body • bronchopulmonary dysplasia • thyroid transcription factor 1 • immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Matsuzaki, V. Besnard, J. C. Clark, Y. Xu, S. E. Wert, M. Ikegami, and J. A. Whitsett
STAT3 Regulates ABCA3 Expression and Influences Lamellar Body Formation in Alveolar Type II Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 551 - 558.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Besnard, Y. Xu, and J. A. Whitsett
Sterol response element binding protein and thyroid transcription factor-1 (Nkx2.1) regulate Abca3 gene expression
Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1395 - L1405.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Cheong, H. Zhang, M. Madesh, M. Zhao, K. Yu, C. Dodia, A. B. Fisher, R. C. Savani, and H. Shuman
ABCA3 Is Critical for Lamellar Body Biogenesis in Vivo
J. Biol. Chem., August 17, 2007; 282(33): 23811 - 23817.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Bush
Update in Pediatric Lung Disease 2006
Am. J. Respir. Crit. Care Med., March 15, 2007; 175(6): 532 - 540.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Tian, R. Zhou, J. E. Rehg, and S. Jackowski
Role of Phosphocholine Cytidylyltransferase {alpha} in Lung Development
Mol. Cell. Biol., February 1, 2007; 27(3): 975 - 982.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007