This Article Full Text Full Text (PDF) All Versions of this Article: jhc.6A7128.2007v1 55/10/1027    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Archanco, M. Articles by Burrell, M. A. Search for Related Content PubMed PubMed Citation Articles by Archanco, M. Articles by Burrell, M. A. Social Bookmarking                      What's this?

Expression of Leptin and Adiponectin in the Rat Oviduct

Marta Archanco, Javier Gómez-Ambrosi, Manuel Tena-Sempere, Gema Frühbeck and María A. Burrell

Department of Histology and Pathology (MA,MAB) and Metabolic Research Laboratory (JG-A,GF), University of Navarra, Pamplona, Spain; Department of Endocrinology, Clínica Universitaria, Pamplona, Spain (GF); and Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, Spain (MT-S)

Correspondence to: Dr. Marta Archanco, Department of Histology and Pathology, University of Navarra, c/Irunlarrea 1, 31008, Pamplona, Spain. E-mail: marclab{at}alumni.unav.es

In mammals, the oviduct is an important source of factors that play key roles in reproductive and developmental events. The major components of oviduct fluid are oviduct-specific glycoproteins, but other proteins are synthesized and secreted by the oviduct epithelium. Leptin and adiponectin are two hormones originally identified in adipocytes that play a critical role not only in the control of energy balance and metabolism but also in diverse functions such as reproduction. This study investigates the presence and distribution of leptin and adiponectin in the rat oviduct through a combination of immunohistochemistry and reverse transcription–polymerase chain reaction techniques. Using both techniques, it has been detected that the oviduct of cycling rats expresses leptin and adiponectin. Immunoreactivity for both adipokines appears in the apical region of the secretory epithelial cells, only in the isthmus and ampulla. The immunostain is stronger in the isthmus and changes throughout the estrous cycle in the ampulla, increasing from proestrous to estrous. The results presented here are a further contribution to the identification of leptin and adiponectin produced and secreted by the oviduct epithelium, which must be taken into account for a better understanding of the reproductive events that take place in this organ. (J Histochem Cytochem 55:1027–1037, 2007)

Key Words: leptin • adiponectin • adipokines • rat oviduct • estrous cycle • secretory cells • oocyte • immunohistochemistry • reverse transcriptase–polymerase chain reaction

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. E. Caminos, R. Nogueiras, F. Gaytan, R. Pineda, C. R. Gonzalez, M. L. Barreiro, J. P. Castano, M. M. Malagon, L. Pinilla, J. Toppari, et al. Novel Expression and Direct Effects of Adiponectin in the Rat Testis Endocrinology, July 1, 2008; 149(7): 3390 - 3402. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007