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Originally published as JHC exPRESS on August 21, 2007.
doi:10.1369/jhc.7A7274.2007
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Journal of Histochemistry and Cytochemistry
Volume 55 (12): 1229-1235, 2007
Copyright ©The Histochemical Society, Inc.

Distribution of Endothelin-converting Enzyme-1 and Neutral Endopeptidase in Human Endometrium

Akira Iwase, Hisao Ando, Tetsuro Nagasaka, Maki Goto, Toko Harata and Fumitaka Kikkawa

Department of Obstetrics and Gynecology (AI,HA,MG,TH,FK) and Department of Maternal and Perinatal Medicine (AI,MG,TH), Nagoya University Graduate School of Medicine, Nagoya, Japan, and Division of Pathology, Clinical Laboratory, Nagoya University Hospital, Nagoya, Japan (TN)

Correspondence to: Dr. Akira Iwase, Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. E-mail: akiwase{at}med.nagoya-u.ac.jp

The expression of endothelin-1 (ET-1), which has been proposed to have a potential autocrine/paracrine role, varies during the menstrual cycle, and therefore, ET-1 may be involved in the cyclic change of the human endometrium. However, neither the synthesis nor the degradation of ET-1 in the endometrium has been determined in detail. We investigated endothelin-converting enzyme-1 (ECE-1), which converts big-ET-1 to active ET-1, and neutral endopeptidase (NEP), which cleaves and inactivates ET-1 in human endometrium in vivo and in vitro. Western blot analysis demonstrated that the change in the expression of ECE-1 during the menstrual cycle differed from that of NEP in the endometrium. ECE-1 was expressed by endometrial epithelial cells, whereas NEP was predominantly expressed by stromal cells in vivo and in vitro. In conclusion, our results suggest that spacio-temporal expression of two endopeptidases, ECE-1 and NEP, involved in the synthesis and degradation of ET-1, might regulate ET-1 action in human endometrium. (J Histochem Cytochem 55:1229–1235, 2007)

Key Words: ECE-1 • endometrium • ET-1 • NEP


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