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Distribution of Pancreatic Endocrine Cells Including IAPP-expressing Cells in Non-diabetic and Type 2 Diabetic Cases

Katsumichi Iki and Parviz M. Pour

UNMC Eppley Cancer Center (KI,PMP) and Department of Pathology and Microbiology (PMP), University of Nebraska Medical Center, Omaha, Nebraska

Correspondence to: Parviz M. Pour, MD, 986805 Nebraska Medical Center, Omaha, NE 68198-6805. E-mail: ppour{at}unmc.edu

There is a lack of agreement on the distribution of islet amyloid polypeptide (IAPP) in the pancreases of healthy and diabetic subjects. Therefore, a detailed morphometrical and immunohistochemical study was performed to obtain information on the distribution of cells expressing insulin, glucagon, somatostatin, pancreatic polypeptide (PP), and IAPP in the pancreases of non-diabetic (n=4) and diabetic individuals (n=6). In the non-diabetic cases, ß-cells contributed to 64%, -cells to 26%, -cells to 8%, PP cells to 0.3%, and IAPP cells to 34% of the islet cell population. The ratio of IAPP/insulin was 1:2. In diabetic cases, ß-cells were decreased by 24%, and IAPP was decreased by 57%. The - and -cells were increased by 40% and 58%, respectively. IAPP/insulin ratio was decreased by 41%. Thus, only 50% of the ß-cells in non-diabetics and only 30% in diabetics coexpressed IAPP. In diabetics, more -cells coexpressed IAPP than in non-diabetics. The results seem to argue against the notion that the secretion of IAPP is increased in diabetics. It is possible that an increase in somatostatin and glucagon plays a greater role in diabetes than IAPP. (J Histochem Cytochem 55:111–118, 2007)

Key Words: diabetes • islets • islet amyloid polypeptide • morphometry • immunohistochemistry

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