This Article Full Text Full Text (PDF) All Versions of this Article: jhc.6A7020.2006v1 55/3/209    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kulbatski, I. Articles by Tator, C. H. Search for Related Content PubMed PubMed Citation Articles by Kulbatski, I. Articles by Tator, C. H. Social Bookmarking                      What's this?

Oligodendrocytes and Radial Glia Derived From Adult Rat Spinal Cord Progenitors: Morphological and Immunocytochemical Characterization

Iris Kulbatski, Andrea J. Mothe, Armand Keating, Yoji Hakamata, Eiji Kobayashi and Charles H. Tator

Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada (IK,AK,CHT); Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada (IK,AJM,CHT); Princess Margaret Hospital/Ontario Cancer Institute, Toronto, Ontario, Canada (AK); and Divisions of Organ Replacement Research and Animal Resource Project, Centre for Molecular Medicine, Jichi Medical School, Kawachi, Tochigi, Japan (YH,EK)

Correspondence to: Iris Kulbatski, BSc (Hons), MSc, Toronto Western Hospital, McL 12-423, 399 Bathurst Street, Toronto, ON M5T 2S8, Canada. E-mail: iris.kulbatski{at}sympatico.ca

Self-renewing, multipotent neural progenitor cells (NPCs) reside in the adult mammalian spinal cord ependymal region. The current study characterized, in vitro, the native differentiation potential of spinal cord NPCs isolated from adult enhanced green fluorescence protein rats. Neurospheres were differentiated, immunocytochemistry (ICC) was performed, and the positive cells were counted as a percentage of Hoescht+ nuclei in 10 random fields. Oligodendrocytes constituted most of the NPC progeny (58.0% of differentiated cells; 23.4% in undifferentiated spheres). ICC and electron microscopy (EM) showed intense myelin production by neurospheres and progeny. The number of differentiated astrocytes was 18.0%, but only 2.8% in undifferentiated spheres. The number of differentiated neurons was 7.4%, but only 0.85% in undifferentiated spheres. The number of differentiated radial glia (RG) was 73.0% and in undifferentiated spheres 80.9%. EM showed an in vitro phagocytic capability of NPCs. The number of undifferentiated NPCs was 32.8% under differentiation conditions and 78.9% in undifferentiated spheres. Compared with ependymal region spheres, the spheres derived from the peripheral white matter of the spinal cord produced glial-restricted precursors. These findings indicate that adult rat spinal cord ependymal NPCs differentiate preferentially into oligodendrocytes and RG, which may support axonal regeneration in future trials of transplant therapy for spinal cord injury. (J Histochem Cytochem 55:209–222, 2007)

Key Words: spinal cord injury • neural progenitors • regeneration • differentiation • phenotypic characterization • oligodendrocyte • radial glia

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. Kulbatski and C. H. Tator Region-specific Differentiation Potential of Adult Rat Spinal Cord Neural Stem/Precursors and Their Plasticity in Response to In Vitro Manipulation J. Histochem. Cytochem., May 1, 2009; 57(5): 405 - 423. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007