This Article Full Text Full Text (PDF) Supplemental Data for this article All Versions of this Article: jhc.6A7152.2007v1 55/6/597    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maleszewski, J. Articles by Halushka, M. K. Search for Related Content PubMed PubMed Citation Articles by Maleszewski, J. Articles by Halushka, M. K. Social Bookmarking                      What's this?

Robust Immunohistochemical Staining of Several Classes of Proteins in Tissues Subjected to Autolysis

Joseph Maleszewski, Jie Lu, Karen Fox-Talbot and Marc K. Halushka

Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland

Correspondence to: Marc K. Halushka, MD, PhD, Ross Building, RM 632L, 720 Rutland Avenue, Baltimore, MD 21205. E-mail: mhalush1{at}jhmi.edu

Despite the common use of immunohistochemistry in autopsy tissues, the stability of most proteins over extended time periods is unknown. The robustness of signal for 16 proteins (MMP1, MMP2, MMP3, MMP9, TIMP1, TIMP2, TIMP3, AGER, MSR, SCARB1, OLR1, CD36, LTF, LGALS3, LYZ, and DDOST) and two measures of advanced glycation end products (AGE, CML) was evaluated. Two formalin-fixed, paraffin-embedded human tissue arrays containing 16 tissues each were created to evaluate 48 hr of autolysis in a warm or cold environment. For these classes of proteins, matrix metalloproteinases and their inhibitors, scavenger receptors, and advanced glycation end product receptors, we saw no systematic diminution of signal intensity during a period of 24 hr. Analysis was performed by two independent observers and confirmed for a subset of proteins by digital analysis and Western blotting. We conclude that these classes of proteins degrade slowly and faithfully maintain their immunohistochemistry characteristics over at least a 24-hr time interval in devitalized tissues. This study supports the use of autopsy tissues with short postmortem intervals for immunohistochemical studies for diseases such as diabetic vascular disease, cancer, Alzheimer's disease, atherosclerosis, and other pathological states. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 55:597–606, 2007)

Key Words: immunohistochemistry • autopsy • autolysis • protein stability

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. K. Halushka, E. Selvin, J. Lu, A. M. Macgregor, and T. C. Cornish Use of Human Vascular Tissue Microarrays for Measurement of Advanced Glycation Endproducts J. Histochem. Cytochem., June 1, 2009; 57(6): 559 - 566. [Abstract] [Full Text] [PDF]

				A. M. Macgregor, C. G. Eberhart, M. Fraig, J. Lu, and M. K. Halushka Tissue Inhibitor of Matrix Metalloproteinase-3 Levels in the Extracellular Matrix of Lung, Kidney, and Eye Increase With Age J. Histochem. Cytochem., March 1, 2009; 57(3): 207 - 213. [Abstract] [Full Text] [PDF]

				L. Lyck, I. Dalmau, J. Chemnitz, B. Finsen, and H. D. Schroder Immunohistochemical Markers for Quantitative Studies of Neurons and Glia in Human Neocortex J. Histochem. Cytochem., March 1, 2008; 56(3): 201 - 221. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007