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Studies on the Expression of 80-kDa Human Sperm Antigen in Rat Testis and Epididymis

Bharati G. Khobarekar, Vandana J. Vernekar, Esakki Prabagaran, Vijaya P. Raghavan and Atmaram H. Bandivdekar

National Institute for Research in Reproductive Health (ICMR), Mumbai, India

Correspondence to: Atmaram H. Bandivdekar, Assistant Director, Division of Biochemistry, National Institute for Research in Reproductive Health (ICMR), JM Street, Parel, Mumbai, 400 012, India. E-mail: batmaram{at}gmail.com

The 80-kDa human sperm antigen (HSA) has demonstrated to be a promising candidate for development of an antifertility vaccine because it is a sperm-specific, conserved, and immunogenic protein. The present study demonstrates the androgen-regulated expression of 80-kDa HSA in testis and epididymis of rat by immunohistochemistry (IHC), using its specific antibodies. Developmental expression of 80-kDa HSA was investigated on days 10, 20, 40, 60, and 90 of age in the testis and epididymis by IHC, and relative staining intensity was estimated by image analysis using BIOVIS software. On days 10 and 20, no significant staining was observed in the testis and epididymis, whereas it gradually increased from day 40 onwards. The highest staining was seen on day 90 in both testis and epididymis. Gradual increase in expression of 80-kDa HSA after day 40 suggests that it is possibly regulated by androgen. To study the androgen-regulated expression of 80-kDa, adult male rats were treated with 75 mg/kg body weight of ethylene dimethane sulfonate (EDS), which selectively destroys Leydig cells and thus induces complete androgen withdrawal. It was observed that the staining intensity decreased following EDS treatment in rat testis as well as epididymis, and it was regained after supplementation with dihydrotestosterone. Increased expression during sexual maturation at the time of testosterone surge and its regulation by antiandrogen/androgen treatment suggest androgen-dependent expression of 80-kDa HSA in rat testis and epididymis. (J Histochem Cytochem 55:753–762, 2007)

Key Words: sperm antigen • developmental expression • androgen • antiandrogen • immunohistochemistry • immunocontraception

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