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Originally published as JHC exPRESS on April 16, 2007.
doi:10.1369/jhc.7A7185.2007
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Journal of Histochemistry and Cytochemistry
Volume 55 (9): 877-883, 2007
Copyright ©The Histochemical Society, Inc.

Immunohistochemical Expression of RAR{alpha}, RARß, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Nasséra Chalabi, Laetitia Delort, Samir Satih, Pierre Déchelotte, Yves-Jean Bignon and Dominique J. Bernard-Gallon

Département d'Oncogénétique, Centre Jean Perrin, Clermont-Ferrand, France (NC,LD,SS,Y-JB,DJB-G); INSERM UMR 484, Clermont-Ferrand, France (NC,LD,SS,Y-JB,DJB-G); Centre de Recherche en Nutrition Humaine, Clermont-Ferrand, France (NC,LD,SS,Y-JB,DJB-G); Anatomie Pathologique, Hôtel Dieu, Clermont-Ferrand, France (PD); and Université d'Auvergne, Clermont-Ferrand, France (PD,Y-JB)

Correspondence to: Pr Yves-Jean Bignon, Département d’Oncogénétique, Centre Jean Perrin, UMR 484 INSERM-UdA, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand Cedex 1, France. E-mail: Yves-Jean.BIGNON{at}cjp.fr

Lycopene, the major carotenoid found in tomatoes, is a potent antioxidant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RAR{alpha}, RARß, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 µM lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RAR{alpha} and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines. (J Histochem Cytochem 55:877–883, 2007)

Key Words: lycopene • breast cancer cell lines • immunohistochemistry • RT-QPCR • RAR{alpha} • RARß • Cx43


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