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Originally published as JHC exPRESS on October 15, 2007.
doi:10.1369/jhc.7A7281.2007
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Journal of Histochemistry and Cytochemistry
Volume 56 (1): 67-75, 2008
Copyright ©The Histochemical Society, Inc.

Expression and Localization of Aquaporins in the Kidney of the Musk Shrew (Suncus murinus)

Seishi Maeda, Sachi Kuwahara, Hisao Ito, Koichi Tanaka, Tetsu Hayakawa and Makoto Seki

Department of Anatomy (SM,SK,KT,TH,MS) and The Institute of Laboratory Animal Science (HI), Hyogo College of Medicine, Hyogo, Japan

Correspondence to: Seishi Maeda, PhD, Dept. of Anatomy, Hyogo College of Medicine, Mukogawa 1-1, Nishinomiya, Hyogo 663-8501, Japan. E-mail: maedas{at}hyo-med.ac.jp

Expression and localization of members of the aquaporin (AQP) family (AQP1, 2, 3, 4, and 5) in the kidney of the musk shrew (Suncus murinus) was examined by immunohistochemistry. AQP1 was expressed in the proximal tubules and in the thin limb of the loops of Henle. AQP1 was the only water channel expressed in the proximal nephron examined, indicating that AQP1 may be an independent water transporter in the proximal nephron. AQP2 and AQP5 were localized to the apical cytoplasm of the cortical to medullary collecting duct (CD) cells and AQP3 and AQP4 were localized to the basal aspect of the cortical to medullary CD cells. AQP3 expression was weaker in the cortical cells compared with the medullary cells, whereas AQP4 was strongly positive throughout the CD. These indicate that the CD is the main water reabsorption segment of the nephron and is regulated by AQPs. Indeed, apical water transport of CD cells of the musk shrew may be controlled by both AQP2 and AQP5. The characteristic expression pattern of the AQPs in this animal provides a novel animal model for elucidating the regulation of water reabsorption by AQPs in the mammalian kidney. (J Histochem Cytochem 56:67–75, 2008)

Key Words: aquaporin • immunohistochemistry • insectivore • Suncus • urine • water channel • water reabsorption


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