Journal of Histochemistry and Cytochemistry
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Originally published as JHC exPRESS on September 2, 2008.
doi:10.1369/jhc.2008.951780
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Journal of Histochemistry and Cytochemistry
Volume 56 (12): 1093-1098, 2008
Copyright ©The Histochemical Society, Inc.

Distribution of Tight Junction Proteins in Adult Human Salivary Glands

Ola M. Maria, Jung-Wan Martin Kim, Jonathan A. Gerstenhaber, Bruce J. Baum and Simon D. Tran

Faculty of Dentistry, McGill University, Montreal, Canada (OMM,J-WMK,SDT), and National Institute of Dental and Craniofacial Research, Bethesda, Maryland (JAG,BJB)

Correspondence to: Simon Tran, 3640 University Street, Room M43, Montreal, Quebec, Canada H3A 2B2. E-mail: simon.tran{at}mcgill.ca

Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to develop an orally implantable artificial salivary gland that would be suitable for treating patients lacking salivary parenchymal tissue. To date, little is known about the distribution of TJ proteins in adult human salivary cells and thus what key molecular components might be desirable for the cellular component of an artificial salivary gland device. Therefore, the aim of this study was to determine the distribution of TJ proteins in human salivary glands. Salivary gland samples were obtained from 10 patients. Frozen and formalin-fixed paraffin-embedded sections were stained using IHC methods. Claudin-1 was expressed in ductal, endothelial, and ~25% of serous cells. Claudins-2, -3, and -4 and JAM-A were expressed in both ductal and acinar cells, whereas claudin-5 was expressed only in endothelial cells. Occludin and ZO-1 were expressed in acinar, ductal, and endothelial cells. These results provide new information on TJ proteins in two major human salivary glands and should serve as a reference for future studies to assess the presence of appropriate TJ proteins in a tissue-engineered human salivary gland. (J Histochem Cytochem 56:1093–1098, 2008)

Key Words: salivary gland • tight junction • claudins • occludin • junctional adhesion molecules • zonula occludens • epithelial barriers


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