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Class III β-Tubulin Is a Component of the Mitotic Spindle in Multiple Cell Types

Eeva-Mari Jouhilahti, Sirkku Peltonen and Juha Peltonen

Institute of Biomedicine, Department of Cell Biology and Anatomy (E-MJ,JP) and Department of Dermatology (JP,SP), University of Turku, Turku, Finland

Correspondence to: Juha Peltonen, Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland. E-mail: juha.peltonen{at}utu.fi

The findings of this study show that Class III β-tubulin is a component of the mitotic spindle in multiple cell types. Class III β-tubulin has been widely used as a neuron-specific marker, but it has been detected also in association with breast and pancreatic cancers. In this study, we describe a novel finding of Class III β-tubulin in a subpopulation of cells in malignant peripheral nerve sheath tumor. The findings of this study also show that Class III β-tubulin is expressed by normal mesenchymal and epithelial cells (fibroblasts and keratinocytes), two transitional cell carcinoma cell lines, and neurofibroma Schwann cells, as shown by immunolabeling and Western transfer analysis using two different Tuj-1 antibodies that are specific for Class III β-tubulin. The corresponding mRNA was detected using RT-PCR and whole human genome microarrays. Both antibodies localized Class III β-tubulin to the mitotic spindle and showed a colocalization with -tubulin. The immunoreaction became visible in early prophase, and the most intense immunoreaction was detected during metaphase and anaphase when microtubules were connected to the kinetochores on chromosomes. Class III β-tubulin–specific immunoreaction lasted to the point when the midbody of cytokinesis became detectable. (J Histochem Cytochem 56:1113–1119, 2008)

Key Words: Class III β-tubulin • malignancy • mitosis • malignant peripheral nerve sheath tumor • neurofibromas • TUBB3 • Tuj1

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