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Originally published as JHC exPRESS on November 12, 2007.
doi:10.1369/jhc.7A7365.2007
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Journal of Histochemistry and Cytochemistry
Volume 56 (2): 183-191, 2008
Copyright ©The Histochemical Society, Inc.

Pancreatic Islet Immunoreactivity to the Reg Protein INGAP

David A. Taylor-Fishwick, Angela Bowman, MariCarmen Korngiebel-Rosique and Aaron I. Vinik

Departments of Medicine (DAT-F,AB,MK-R,AIV), Microbiology and Molecular Cell Biology (DAT-F), Anatomy and Pathology (AIV), Eastern Virginia Medical School, Norfolk, Virginia

Correspondence to: David A. Taylor-Fishwick, PhD, Director, Cell and Molecular Biology, Diabetes Research Institute, EVMS, 855 W. Brambleton Ave., Norfolk, VA. E-mail: Taylord{at}evms.edu

The Reg-related protein family member INGAP (islet neogenesis-associated protein) is a pleiotropic factor enhancing islet neogenesis, neurite growth, β-cell protection, and β-cell function. Using an antibody to the N-termini of INGAP, we have identified that immunoreactivity to INGAP localized to the pancreatic endocrine cells in mouse. INGAP- and insulin-immunoreactive cells are mutually exclusive, with INGAP-immunoreactive cells being preserved after streptozotocin-mediated destruction of β-cells. Glucagon- and INGAP-immunoreactive cells colocalize, although respective antigen expression occurs in different intracellular locations. These data suggest that INGAP-immunoreactive cells include {alpha}-cells; however, detection of single INGAP-immunoreactive/glucagon-negative cells indicates that this may not be exclusive. In addition to mouse, detection of islet endocrine cells that were INGAP immunoreactive/glucagon immunoreactive/insulin negative was also observed in islets from human, monkey, and rat. These findings reveal that INGAP and/or related group 3 Reg proteins have a conserved expression in the pancreatic islet. (J Histochem Cytochem 56:183–191, 2008)

Key Words: islet • glucagon • islet neogenesis-associated protein


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