Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
Originally published as JHC exPRESS on November 26, 2007.
doi:10.1369/jhc.7A7310.2007
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.7A7310.2007v1
56/3/275    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Bowen, K. B.
Right arrow Articles by Oxford, J. T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bowen, K. B.
Right arrow Articles by Oxford, J. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 56 (3): 275-283, 2008
Copyright ©The Histochemical Society, Inc.

Immunohistochemical Localization of Collagen Type XI {alpha}1 and {alpha}2 Chains in Human Colon Tissue

Kara B. Bowen, Aaron P. Reimers, Sarah Luman, Joseph D. Kronz, William E. Fyffe and Julia Thom Oxford

Department of Biology, Northwest Nazarene University, Nampa, Idaho (KBB,APR,SL,WEF); Department of Pathology, Mercy Medical Center, Nampa, Idaho (JDK); and Department of Biology, Biomolecular Research Center, Boise State University, Boise, Idaho (JTO)

Correspondence to: Julia Thom Oxford, Department of Biology, Biomolecular Research Center, Boise State University, 1910 University Drive, Boise, ID 83725. E-mail: joxford{at}boisestate.edu

In previous studies, collagen XI mRNA has been detected in colon cancer, but its location in human colon tissue has not been determined. The heterotrimeric collagen XI consists of three {alpha} chains. While it is known that collagen XI plays a regulatory role in collagen fibril formation, its function in the colon is unknown. The characterization of normal human colon tissue will allow a better understanding of the variance of collagen XI in abnormal tissues. Grossly normal and malignant human colon tissue was obtained from pathology archives. Immunohistochemical staining with a 58K Golgi marker and {alpha}1(XI) and {alpha}2(XI) antisera was used to specifically locate their presence in normal colon tissue. A comparative bright field microscopic analysis showed the presence of collagen XI in human colon. The juxtanuclear, dot-like collagen XI staining in the Golgi apparatus of goblet cells in normal tissue paralleled the staining of the 58K Golgi marker. Ultra light microscopy verified these results. Staining was also confirmed in malignant colon tissue. This study is the first to show that collagen XI is present in the Golgi apparatus of normal human colon goblet cells and localizes collagen XI in both normal and malignant tissue. Although the function of collagen XI in the colon is unknown, our immunohistochemical characterization provides the foundation for future immunohistopathology studies of the colon. (J Histochem Cytochem 56:275–283, 2008)

Key Words: collagen type XI • colon • immunohistochemistry • Golgi apparatus • 58K Golgi marker


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008