This Article Full Text Full Text (PDF) All Versions of this Article: jhc.7A7359.2008v1 56/5/433    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chen, X. Articles by Dove, W. F. Search for Related Content PubMed PubMed Citation Articles by Chen, X. Articles by Dove, W. F. Social Bookmarking                      What's this?

Cellular Expression Patterns of Genes Upregulated in Murine and Human Colonic Neoplasms

Xiaodi Chen, William M. Ehrhardt, Richard B. Halberg, Bruce J. Aronow and William F. Dove

McArdle Laboratory for Cancer Research (XC,WME,RBH,WFD) and Laboratory of Genetics (WFD), University of Wisconsin, Madison, Wisconsin, and Division of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio (BJA)

Correspondence to: William F. Dove, McArdle Laboratory for Cancer Research, 1400 University Avenue, Madison, WI 53706. E-mail: dove{at}oncology.wisc.edu

Markers overexpressed in colonic tumors of the multiple intestinal neoplasia (Min) mouse have been recently identified by cDNA subtractive hybridization and by microarray analysis. The significance of such a marker depends on its expression in tumor vs stromal lineages and on its expression pattern in normal tissue. From 34 differentially expressed markers, 14 were found to be expressed from supporting lineages. The markers expressed in the tumor lineage were grouped into three classes on the basis of ISH in mouse models and IHC in human adenomas. The first class includes markers expressed both in neoplastic cells and in the proliferating cells residing at the bottom of normal colonic crypts. The second class of markers shows elevated expression in neoplastic cells and also in the postmitotic Paneth cells of the small intestine. Finally, the third class of marker shows detectable intestinal expression only within tumors but not in the normal intestinal epithelium. Is such a tumor-associated marker uniquely essential for tumor growth? Deficiency for the tumor-associated glycoprotein clusterin does not affect the multiplicity or growth rate of intestinal tumors in Min mice. Thus, clusterin is a candidate secreted colon cancer marker but not a single target for chemoprevention or therapy. (J Histochem Cytochem 56:433–441, 2008)

Key Words: colorectal cancer • gene expression • Min mouse • intestinal tumor • clusterin

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008