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Originally published as JHC exPRESS on February 18, 2008.
doi:10.1369/jhc.2008.950741
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Journal of Histochemistry and Cytochemistry
Volume 56 (5): 511-516, 2008
Copyright ©The Histochemical Society, Inc.

Cannabinoid CB1 Receptors Are Expressed by Parietal Cells of the Human Gastric Mucosa

María Ruth Pazos, Rosa María Tolón, Cristina Benito, Conrado Fernández Rodríguez, Juan José Gorgojo, Manuel Nevado, Mariano Álvarez, Francisco Arias, Francisca Almodóvar, María Teresa Pérez Fernández, José Luis Lledó, Sara González, José Javier Fernández-Ruiz and Julián Romero

Laboratory of Research and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED) (MRP,RMT,CB,JR), Unit of Gastroenterology (CFR,MTPF,JLL), Unit of Endocrinology (JJG,FAlmodóvar), Unit of Pathology (MN), Unit of Surgery (MÁ), and Unit of Psychiatry (FArias), Fundación Hospital Alcorcón, Alcorcón, Madrid, Spain, and Department of Biochemistry and Molecular Biology, Faculty of Medicine, and CIBERNED, Complutense University, Madrid, Spain (SG,JJF-R)

Correspondence to: Julián Romero, PhD, Laboratorio de Apoyo a la Investigación, Fundación Hospital Alcorcón, C/ Budapest 1, 28922 Alcorcón, Madrid, Spain. E-mail: jromerop{at}fhalcorcon.es

Experimental data suggest that the endogenous cannabinoid system is involved in gastric function in different animal species. In most of them, CB1 receptors have been localized on vagal terminals innervating the external wall of the stomach. We aimed at studying the putative presence and distribution of these receptors in the human gastric mucosa. To this end, we first performed Western blotting, RT-PCR, in situ hybridization, and immunohistochemical analysis of CB1 protein distribution in biopsy samples of healthy individuals. To determine the precise cell populations expressing CB1 receptors, we performed double immunofluorescence plus confocal microscopy analysis of the same samples. Our results show that CB1 receptors are present in the gastric epithelium of the mucosa. Specifically, they are expressed by a subpopulation of mucosal cells, the acid-secreting parietal cells, as shown by double immunohistochemical staining and by their differential abundance in subregions of the gastric mucosa. These results reinforce the notion of a prominent role for the endocannabinoid system in the gastric function in humans and postulate the use of cannabinoid CB1 receptors in parietal cells as new therapeutic targets for the regulation of gastric acid production. (J Histochem Cytochem 56:511–516, 2008)

Key Words: cannabinoids • receptor • acid secretion • parietal cell


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