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Originally published as JHC exPRESS on March 3, 2008.
doi:10.1369/jhc.7A7354.2008
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Journal of Histochemistry and Cytochemistry
Volume 56 (6): 605-614, 2008
Copyright ©The Histochemical Society, Inc.

Glomerular CD34 Expression in Short- and Long-term Diabetes

Luz Marina Acevedo1, Irene Londono1, Malika Oubaha, Lucian Ghitescu and Moise Bendayan

Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada

Correspondence to: Moise Bendayan, Department of Pathology and Cell Biology, Université de Montréal, 2900 Edouard Montpetit, Pav. Roger Gaudry, Room R-810, Montréal QC H3T 1J4, Canada. E-mail: moise.bendayan{at}umontreal.ca

Aging and diabetes are associated with exacerbated expression of adhesion molecules. Given their importance in endothelial dysfunction and their possible involvement in the alteration of glomerular permeability occurring in diabetes, we have evaluated expression of the sialomucin-type adhesion molecule CD34 in renal glomerular cells of normal and diabetic animals at two different ages by colloidal gold immunocytochemistry and immunoblotting. CD34 labeling was mostly assigned to the plasma membranes of glomerular endothelium and mesangial processes. Podocyte membranes were also labeled, but to a lesser degree. Short- and long-term diabetes triggers a substantial increase in immunogold labeling for CD34 in renal tissues compared with young normoglycemic animals. However, the level of labeling in old diabetic and healthy control rats is similar, suggesting that the effect of diabetes and aging on CD34 expression is similar but not synergistic. Western blotting of isolated glomerular fractions corroborated immunocytochemical results. Increased expression of CD34 may reflect its involvement in the pathogenesis of glomerular alterations related to age and diabetes. Alterations present in early diabetes, resembling those occurring with age, strengthen the concept that diabetes is an accelerated form of aging.(J Histochem Cytochem 56:605–614, 2008)

Key Words: CD34 • aging • diabetes • glomerular wall • immunocytochemistry • sialomucin • adhesion molecule


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