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A Guide to the Perplexed on the Specificity of Antibodies

Clifford B. Saper

Department of Neurology and Program in Neuroscience, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, Massachusetts

Correspondence to: Clifford B. Saper, MD, PhD, Department of Neurology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215. E-mail: csaper{at}bidmc.harvard.edu

Summary

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation. (J Histochem Cytochem 57:1–5, 2009)

Key Words: immunohistochemistry • immunocytochemistry • controls

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