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Originally published as JHC exPRESS on September 15, 2008.
doi:10.1369/jhc.2008.951582
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Journal of Histochemistry and Cytochemistry
Volume 57 (1): 17-27, 2009
Copyright ©The Histochemical Society, Inc.

Oat5 and NaDC1 Protein Abundance in Kidney and Urine After Renal Ischemic Reperfusion Injury

Gisela Di Giusto, Naohiko Anzai, Hitoshi Endou and Adriana M. Torres

Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Rosario, Argentina (GDG,AMT), and Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan (NA,HE)

Correspondence to: Adriana Mónica Torres, PhD, Professor of Pharmacology, Suipacha 531 City, Rosario 2000, Argentina. E-mail: admotorres{at}yahoo.com.ar

The aim of this study was to evaluate the abundance of the organic anion transporter 5 (Oat5) and the sodium-dicarboxylate cotransporter 1 (NaDC1) in kidney and urine after renal ischemic reperfusion injury. Renal injury was induced in male Wistar rats by occlusion of both renal pedicles for 0 (Group Sham), 5 (Group I5R60), or 60 (Group I60R60) min. The studies were performed after 60 min of reperfusion. The expression of Oat5 and NaDC1 was evaluated by IHC and Western blotting. Oat5 and NaDC1 abundance and alkaline phosphatase activity (AP) were assayed in urine. A decreased expression in renal homogenates and apical membranes and an increase in urinary excretion of Oat5 and NaDC1 were observed in I60R60 rats, as well as alterations of other widely used parameters for renal dysfunction and injury (plasma creatinine, urinary AP activity, kidney weight, histological lesions). In contrast, in the I5R60 group, only an increase in urinary excretion of Oat5 and mild histopathological damage was detected. This is the first study on Oat5 and NaDC1 detection in urine. These results suggest that urinary excretion of Oat5 might be an early indicator of renal dysfunction, which is useful for detection of even minor alterations in renal structural and functional integrity. (J Histochem Cytochem 57:17–27, 2009)

Key Words: acute renal failure • ischemia and reperfusion • Oat5 • NaDC1


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