Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

Originally published as JHC exPRESS on September 29, 2008.
doi:10.1369/jhc.2008.951996
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jhc.2008.951996v1
57/1/51    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lobo, M. V.T.
Right arrow Articles by Martín-Hidalgo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lobo, M. V.T.
Right arrow Articles by Martín-Hidalgo, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 57 (1): 51-60, 2009
Copyright ©The Histochemical Society, Inc.

Hormone-sensitive Lipase Expression and IHC Localization in the Rat Ovary, Oviduct, and Uterus

María V.T. Lobo, Lydia Huerta, María Isabel Arenas, Rebeca Busto, Miguel Angel Lasunción and Antonia Martín-Hidalgo

Department of Cell Biology and Genetics, Alcalá de Henares University, Madrid, Spain (MVTL,MIA); Service of Biochemistry-Research Department, Ramón y Cajal Hospital, Madrid, Spain (LH,RB,MAL,AM-H); and Ciber Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid, Spain (LH,RB,MAL,AM-H)

Correspondence to: Antonia Martín-Hidalgo, PhD, Servicio de Bioquímica-Investigacion, Hospital Ramón y Cajal, Ctra. Colmenar Viejo, Km 9.100, E-28034 Madrid, Spain. E-mail: antonia.martin{at}hrc.es

Hormone-sensitive lipase (HSL) is a key regulator of cholesterol esters metabolism. The aim of this study was to determine HSL localization in rat female reproductive organs during the ovarian cycle by IHC methods. HSL was located in the ovarian epithelium. The granulosa cells and oocytes of primordial follicles were immunonegative. In mature follicles, HSL was found in oocytes and theca and granulosa cells. However, HSL expression in theca cells and oocytes decreased during follicular atresia. Luteal cells showed HSL staining in cytoplasm during proestrus and estrus, in the nucleus during metestrus, and in cytoplasm and the nucleus during diestrus. In the tubaric ampulla, HSL was located in the epithelial cells nuclei and in the cilia during proestrus and estrus but mainly in the nucleus during metestrus and diestrus. In the isthmus, cells showed HSL immunolabeling in the nucleus and cilia during proestrus, but only in the cilia during estrus, metestrus, and diestrus. In the uterus, HSL was found in the epithelial cells nuclei. HSL-immunoreactive bands at 84, 67, 54, and 43 kDa were found in rat female reproductive organs. HSL labeling in the nucleus of epithelial and germ cells suggests an as yet unknown function for this protein, probably related to oogenesis and cell proliferation. (J Histochem Cytochem 57:51–60, 2009)

Key Words: hormone-sensitive lipase • ovary • oviduct • uterus • oogenesis • immunohistochemistry • cholesterol esters


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009

 
Purchase HCS Short Course Manual on HCS site