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Originally published as JHC exPRESS on September 29, 2008.
doi:10.1369/jhc.2008.952499
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Journal of Histochemistry and Cytochemistry
Volume 57 (1): 61-67, 2009
Copyright ©The Histochemical Society, Inc.

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Mariusz T. Skowronski, Tae-Hwan Kwon and Søren Nielsen

Department of Animal Physiology, University of Warmia and Mazury, Olsztyn, Poland (MTS); Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea (THK); and The Water and Salt Research Center, University of Aarhus, Aarhus, Denmark (SN)

Correspondence to: Mariusz T. Skowronski, MVD, PhD, Department of Animal Physiology, University of Warmia and Mazury, Oczapowskiego 1A, 10-718 Olsztyn, Poland. E-mail: skowron{at}uwm.edu.pl

Thirteen mammalian aquaporin (AQP) isoforms have been identified, and they have a unique tissue-specific pattern of expression. AQPs have been documented in the reproductive system of both male and female humans, rats, and mice. However, tissue expression and cellular and subcellular localization of AQPs are unknown in the female reproductive system of pigs. In this study, AQP1 immunoreactivity was detected in the capillary endothelium of the ovary. Distinct immunolabeling of capillary endothelium was also observed in the oviduct and uterus. AQP5 was expressed in flattened follicle cells of primordial follicles, granulosa cells of developing ovarian follicles, and muscle cells of the oviduct and uterus. Staining of AQP5 was also observed in the epithelial cells of the oviduct and uterine epithelium. AQP9 immunoreactivity was observed in granulosa cells of developing follicles. AQP9 was also localized in the luminal epithelial cells of the oviduct and uterine epithelia cells. This is, to our knowledge, the first study that shows tissue expression and cellular and subcellular localization of AQPs in the reproductive system of the female pig. Moreover, these results suggest that several subtypes of the AQPs (AQP1, 5, and 9) are involved in regulation of water homeostasis in the reproductive system of gilts. (J Histochem Cytochem 57:61–67, 2009)

Key Words: aquaporins • immunolocalization • female reproductive system • pig


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