This Article Full Text Full Text (PDF) All Versions of this Article: jhc.2008.951707v1 57/1/69    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ruhin-Poncet, B. Articles by Berdal, A. Search for Related Content PubMed PubMed Citation Articles by Ruhin-Poncet, B. Articles by Berdal, A. Social Bookmarking                      What's this?

Msx and Dlx Homeogene Expression in Epithelial Odontogenic Tumors

Blandine Ruhin-Poncet¹, Sonia Ghoul-Mazgar¹, Dominique Hotton, Frédérique Capron, Mohamed Habib Jaafoura, Gérard Goubin and Ariane Berdal

Laboratory of Orofacial Biology and Pathology–Centre de Recherche des Cordeliers, INSERM, UMR S 872, Team 5, Pierre and Marie Curie University, Paris, France (BR-P,SG-M,DH,GG,AB); Department of Stomatology and Maxillofacial Surgery (BR-P) and Department of Histopathology (FC), Pitié Salpêtrière University Hospital, Paris, France; Laboratory of Histology and Embryology, Dental Faculty of Monastir, Monastir, Tunisia (SG-M); and Laboratory of Histopathology, Orthopedia National Institute of Kassar Said, Mannouba, Tunisia (MHJ)

Correspondence to: Sonia Ghoul-Mazgar, Laboratory of Orofacial Biology and Pathology–Centre de Recherche des Cordeliers, INSERM, UMR S 872, Team 5, Pierre and Marie Curie University, Paris 06, F-75006, 15-21 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. E-mail: ghoulsonia{at}yahoo.fr

Epithelial odontogenic tumors are rare jaw pathologies that raise clinical diagnosis and prognosis dilemmas notably between ameloblastomas and clear cell odontogenic carcinomas (CCOCs). In line with previous studies, the molecular determinants of tooth development—amelogenin, Msx1, Msx2, Dlx2, Dlx3, Bmp2, and Bmp4—were analyzed by RT-PCR, ISH, and immunolabeling in 12 recurrent ameloblastomas and in one case of CCOC. Although Msx1 expression imitates normal cell differentiation in these tumors, other genes showed a distinct pattern depending on the type of tumor and the tissue involved. In benign ameloblastomas, ISH localized Dlx3 transcripts and inconstantly detected Msx2 transcripts in epithelial cells. In the CCOC, ISH established a lack of both Dlx3 and Msx2 transcripts but allowed identification of the antisense transcript of Msx1, which imitates the same scheme of distribution between mesenchyme and epithelium as in the cup stage of tooth development. Furthermore, while exploring the expression pattern of signal molecules by RT-PCR, Bmp2 was shown to be completely inactivated in the CCOC and irregularly noticeable in ameloblastomas. Bmp4 was always expressed in all the tumors. Based on the established roles of Msx and Dlx transcription factors in dental cell fates, these data suggest that their altered expression is a proposed trail to explain the genesis and/or the progression of odontogenic tumors. (J Histochem Cytochem 57:69–78, 2009)

Key Words: epithelial odontogenic tumors • Dlx • Msx • antisense transcript • RT-PCR • in situ hybridization

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009