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Originally published as JHC exPRESS on October 14, 2008.
doi:10.1369/jhc.2008.952283
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Journal of Histochemistry and Cytochemistry
Volume 57 (1): 79-86, 2009
Copyright ©The Histochemical Society, Inc.

Expression of UDP-N-acetyl-D-galactosamine: Polypeptide N-acetylgalactosaminyltransferase-6 in Gastric Mucosa, Intestinal Metaplasia, and Gastric Carcinoma

Joana Gomes, Nuno T. Marcos, Nora Berois, Eduardo Osinaga, Ana Magalhães, João Pinto-de-Sousa, Raquel Almeida, Fátima Gärtner and Celso A. Reis

Institute of Molecular Pathology and Immunology–IPATIMUP (JG,NTM,AM,RA,FG,CAR), Medical Faculty of the University of Porto (JPS,RA,CAR), and Institute of Biomedical Sciences of Abel Salazar–ICBAS (FG), University of Porto, Porto, Portugal, and Departamento de Inmunobiología, Facultad de Medicina, Universidad de la República and Institut Pasteur, Montevideo, Uruguay (NB,EO)

Correspondence to: Celso A. Reis, Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal. E-mail: celso.reis{at}ipatimup.pt

Aberrant mucin O-glycosylation is often observed in cancer and is characterized by the expression of immature simple mucin-type carbohydrate antigens. UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-6 (ppGalNAc-T6) is one of the enzymes responsible for the initial step in O-glycosylation. This study evaluated the expression of ppGalNAc-T6 in human gastric mucosa, intestinal metaplasia, and gastric carcinomas. Our results showed that ppGalNAc-T6 is expressed in normal gastric mucosa and in intestinal metaplasia. A heterogeneous expression and staining pattern for this enzyme was observed in gastric carcinomas. ppGalNAc-T6 was expressed in 79% of the cases, and its expression level was associated with the presence of venous invasion. Our results provide evidence that ppGalNAc-T6 is an IHC marker associated with venous invasion in gastric carcinoma and may contribute to the understanding of the molecular mechanisms that underlie aberrant glycosylation in gastric carcinogenesis and in gastric carcinoma. (J Histochem Cytochem 57:79–86, 2009)

Key Words: mucin O-glycosylation • ppGalNAc-T6 • gastric mucosa • intestinal metaplasia • gastric carcinoma


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