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Immunolocalization of Aquaporin-1, -5, and -7 in the Avian Testis and Vas Deferens

Mariusz T. Skowronski, Anna Leska, Anna Robak and Soren Nielsen

Department of Animal Physiology (MTS,AL) and Department of Anatomy (AR), University of Warmia and Mazury in Olsztyn, Olsztyn, Poland, and The Water and Salt Research Center, University of Aarhus, Aarhus, Denmark (SN)

Correspondence to: Mariusz T. Skowronski, MVD, PhD, University of Warmia and Mazury in Olsztyn, Department of Animal Physiology, Oczapowskiego 1A, 10-718 Olsztyn, Poland. E-mail: skowron{at}uwm.edu.pl

Thirteen mammalian aquaporin (AQP) isoforms have been identified, and they have a unique tissue-specific pattern of expression. AQPs have been found in the reproductive system of both male and female humans, rats, and mice. However, tissue expression and cellular and subcellular localization of AQPs have been poorly investigated in the male reproductive system of birds. The localization of AQP subtypes (AQP1, 2, 3, 4, 5, 7, 8, 9, and 11) in the goose testis and vas deferens has been studied through immunohistochemistry and immunobloting. Interestingly, the testicular and deferential tissues were positive for AQP1, -5, and -7 but not the others. AQP1 immunoreactivity was detected in the capillary endothelial cells of testis and vas deferens. AQP5 was localized in the interstitial tissue of the testis, including Leydig cells, as well as in the basal cells of vas deferens. Double-labeling confocal microscopy revealed coexpression of AQP5 with capillary AQP1 in the testis. AQP7 was expressed in elongated spermatid and spermatozoa tails in the testis, as well as spermatozoa tails in the vas deferens. These results suggest that several subtypes of AQPs are involved in the regulation of water homeostasis in the goose male reproductive system. (J Histochem Cytochem 57:915–922, 2009)

Key Words: aquaporin • immunolocalization • male reproductive system • avian

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