This Article Full Text Full Text (PDF) All Versions of this Article: jhc.2009.953919v1 57/12/1127    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Varecza, Z. Articles by Helyes, Z. PubMed PubMed Citation Articles by Varecza, Z. Articles by Helyes, Z. Social Bookmarking                         What's this?

Expression of the Somatostatin Receptor Subtype 4 in Intact and Inflamed Pulmonary Tissues

Zoltán Varecza, Krisztián Elekes, Terézia László, Anikó Perkecz, Erika Pintér, Zoltán Sándor, János Szolcsányi, Dániel Keszthelyi, Árpád Szabó, Katalin Sándor, Tamás F. Molnár, Zalán Szántó, Judit E. Pongrácz and Zsuzsanna Helyes

Department of Immunology and Biotechnology (ZV,JEP), Department of Pharmacology and Pharmacotherapy (KE,AP,EP,JS,DK,AS,KS,ZH), Department of Pathology (TL), and Department of Surgery (TFM), Faculty of Medicine, University of Pécs, Pécs, Hungary, and Analgesic Research Laboratory of Gedeon-Richter Plc. and the University of Pécs, Pécs, Hungary (ZS)

Correspondence to: Zsuzsanna Helyes, University of Pecs, Faculty of Medicine, Department of Pharmacology and Pharmacotherapy, H-7624 Pecs, Szigeti u. 12, Hungary. E-mail: zsuzsanna.helyes{at}aok.pte.hu

Somatostatin released from capsaicin-sensitive sensory nerves of the lung during endotoxin-induced murine pneumonitis inhibits inflammation and hyperresponsiveness, presumably via somatostatin receptor subtype 4 (sst₄). The goal of the present study was to identify sst₄ receptors in mouse and human lungs and to reveal its inflammation-induced alterations with real-time quantitative PCR, Western blot, and immunohistochemistry. In non-inflamed mouse and human lungs, mRNA expression and immunolocalization of sst₄ are very similar. They are present on bronchial epithelial, vascular endothelial, and smooth-muscle cells. The sst₄ receptor protein in the mouse lung significantly increases 24 hr after intranasal endotoxin administration as well as in response to 3 months of whole-body cigarette smoke exposure, owing to the infiltrating sst₄-positivite mononuclear cells and neutrophils. In the chronically inflamed human lung, the large number of activated macrophages markedly elevate sst₄ mRNA levels, although there is no change in acute purulent pneumonia, in which granulocytes accumulate. Despite mouse granulocytes, human neutrophils do not show sst₄ immunopositivity. We provide the first evidence for the expression, localization, and inflammation-induced alterations of sst₄ receptors in murine and human lungs. Inasmuch as tissue distribution of this receptor is highly similar, extrapolation of murine experimental results to human conditions might be possible. (J Histochem Cytochem 57:1127–1137, 2009)

Key Words: airway inflammation • endotoxin exposure • immunohistochemistry • mouse and human lung pathology • pulmonary macrophage • reverse transcriptase polymerase chain reaction • somatostatin • Western blot

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2009